List of cSABPs identified using a protein microarray

Locus ID Protein name signal ratio FDR SPR with 3AESA crosslinked to 4AzSA [3H]SA binding failed to purify References At3g03900 adenosine-5'-phosphosulfate kinase 3 (APK3) 3.2 0.004 At4g37470 alpha/beta fold hydrolase , Karrikin insensitive 2 (KAI2) 2.60 0.005 At5g19050 alpha/beta-hydrolases superfamily protein 2.17 / 2.31 0.002 / 0.007 Yes (Weak) Yes Ag2g30200 [acyl-carrier-protein] S-malonyltransferases 2.05 / 2.00 0.005 / 0.0001 At1g08250 arogenate dehydratase (ADT6) 4.24 / 3.61 0.0002 / 0.0003 Yes At3g57510 (α) polygalacturonase protein (ADPG1) 1.87 0.004 At4g22820 A20/AN1-like zinc finger family protein; 1.87 0.008 At2g43360 biotin auxotroph 2 (BIO2/BIOB) 3.29 0.009 At3g01500 carbonic anhydrase 1 1.94 0.004 Yes Slaymaker et al., 2002 (in tobacco) At5g12850 CCCH-type zinc finger protein with ARM repeat domain 1.81 0.007 At1g08640 chloroplast J-like domain 1 (CJD1) 1.92 0.007 At5g54340 C2H2 and C2HC zinc fingers superfamily protein 2.39 0.008 Yes At2g44350 citrate synthase 4 (CSY4) 1.67 0.007 At1g33330 Class I peptide chain release factor 2.47 / 2.39 0.002 / 0.002 Yes At3g28760 contains 3-dehydroquinate synthase domain 2.18 0.007 Yes At1g54220 dihydrolipoamide acetyltransferase, long form protein 2.09 0.008 Yes At5g61410 D-ribulose-5-phosphate-3-epimerase (RPE) 2.55 / 2.37 0.002 Yes At1g17940 endosomal targeting BRO1-like domain-containing protein 2 0.003 At1g28570 (ERMO3);(MVP1) ; nuclear cage (NUC) 2.04 0.007 At4g14630 germin-like protein (GLP9) 5.81 / 3.99 0.001 / 0.004 Yes At5g39190 germin-like protein (GLP2a, GER2)) 1.76 0.008 Yes At1g17890 germin-like protein (GER2) 1.95 0.007 At1g06130 glyoxalase 2-4 (GLX2-4) 2.41 0.0008 Yes At1g42970 glyceraldehyde-3-phosphate dehydrogenase B subunit (GAPDHB) 2.90 / 2.53 0.0008 / 0.005 Yes Tian et al., 2015 At1g66200 glutamine synthetase (GSR2) 2.49 0.003 Yes# Yes# At5g35630 glutamine synthetase (GS2) 1.89 0.008 No At2g31570 glutathione peroxidase (GPX2) 2.64 / 2.17 0.003 / 0.007 Yes# Yes# At1g53280 homolog of animal DJ-1 superfamily protein (DJ1B) 2.61 0.003 At1g79870 hydroxypyruvate 2 (HPR2) 2.41 0.008 Yes# Yes# At3g43270 invertase/pectin methylesterase inhibitor superfamily; 3.50 / 2.50 0.002 / 0.006 Yes At3g49220 invertase/pectin methylesterase inhibitor superfamily 3.37 / 3.22 0.0001 / 0.0003 At3g10720 invertase/pectin methylesterase inhibitor superfamily 2.35 0.003 At2g46110 ketopentoate hydroxymethyltransferase 1 (KPGHMT1) 2.84 0.002288148 At5g10920 L-Aspartase-like family protein; argininosuccinate lyase activity 2.18 0.007 At4g09300 LisH and RanBPM domains containing protein 1.77 0.007 At2g22370 mediator 18 (MED18) 1.99 0.007 At1g53240 mitochrondrial malate dehydrogenase (MMDH1) 1.98 0.007 At1g04410 NAD-dependent malate dehydrogenase 1 (C-NAD-MDH1) 2.33 0.006 At5g12040 nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase family protein 2.24 / 2.19 0.005 / 0.005 Yes At3g56520 NAC (No Apical Meristem) domain transcriptional regulator superfamily protein 1.75 0.007 At5g18900 2-oxoglutarate (2OG)/Fe(II)-dependent oxygenase 3.03 0.0003 At1g10640 pectin lyase-like protein 2.55 / 2.44 0.004 / 0.005 At4g33220 pectin methylesterase 44 (PME44) 1.87 0.008 At3g59480 pfkB-like carbohydrate kinase 3.65 0.007 Yes At4g35110 phospholipase-like protein 4 (PEARLI 4) 1.78 0.008 At1g25490 phosphoprotein phosphatase 2A (PP2A) 3.82 / 3.05 0.002/0.002 Yes (Weak) Yes# At1g29410 phosphoribosylanthranilate isomerase 3 (PAI3) 3.25 / 2.12 0.002 / 0.007 Yes At5g50850 pyruvate dehydrogenase, MACCI-BOU (MAB1) 2.59 0.008 At4g33070 pyruvate decarboxylase 1 (PDC1) 2.21 0.006 Yes At1g80360 pyridoxal phosphate (PLP)-dependent transferases (VAS1) 3.15 / 3.10 0.001 / 0.0004 At3g25480 Rhodanese/Cell cycle control phosphatase 2.62 /2.19 0.009 / 0.002 At3g04790 Ribose 5-phosphate isomerase, type A protein 2.45 / 2.42 0.0006 / 0.004 At1g67090 ribulose biphosphate carboxylase small subunit 1A (RBCS1A) 4.60 / 3.89 0.002 / 0.002 Yes# Yes# At5g38410 ribulose bisphosphate small subunit 3B (RBCS3B) 3.92 0.0006 At1g18980 RmlC-like cupins superfamily protein 1.70 0.007 At1g21200 sequence-specific DNA binding transcription factors 1.82 0.007 At1g75980 single hybrid motif superfamily protein 1.86 0.0098 At5g43780 sulfate adenylyltransferase, ATP sulfurylase (APS4) 2.37 / 2.13 0.002 / 0.004 At2g37400 tetratricopeptide repeat (TPR)-like superfamily protein 2.34 0.007 Yes At1g21400 thiamin diphosphate-binding fold (THDP-binding) superfamily protein 2.27 0.0096 Yes At2g44790 uclacyanin 2 (UCC2) 1.70 0.007 At4g23920 UDP-D-glucose/UDP-D-galactose 4-epimerase 2 (UGE2) 3.39 / 3.02 0.0001 / 0.0001 Yes# Yes# At4g10960 UDP-D-glucose/UDP-D-galactose 4-epimerase 5 (UGE5) 2.68 / 2.02 0.003 / 0.004 At2g30140 UDP-Glucosyl transferase 87A2 (UGT87A2) 3.03 0.009 At3g11340 UDP- Glycosyl transferase 76B1 (UGT76B1) 2.96 0.004 At2g29740 UDP-glucosyl transferase 71C2 (UGT71C2) 1.94 0.007 Yes At5g59590 UDP-glucosyl transferase 76E2 (UGT76E2) 1.93 0.007 At1g22400 UDP-glycosyl transferase 85A1 (UGT85A1) 1.70 0.007 At2g34850 UDP-glucose 4-epimerase activity, MEE25) 2.60 0.004 Yes At4g38100 unknown protein (CURT1D) 7.85 / 7.66 0.0001 / 0.0001 Yes At3g51090 unknown function (DUF1640) 1.91 0.002 At1g02540 unknown protein 2.91 / 2.39 0.001 / 0.005 Yes At1g59710 unknown function 1.75 0.007 At1g68140 unknown function 1.57 0.007 At2g35900 unknown protein 2.01 0.005 At2g34470 urease accessory protein G (UREG) 2.36 0.003 At2g14620 xyloglucan endotransglucosylase/hydrolase 10 (XTH10) 2.47 0.003

FDR: false discovery rate signal ratio corresponds to the ratio of fluorescent signal obtained on PMA incubated with 4AzSA divided by the signal obtained on PMA incubated without 4AzSA. # competable with SA