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Plant MetGenMAP manual

Introduction
Input Data Format
Uploading and Processing
    Obtaining an account
    Upload Form
    Data uploading
    Processing Datasets
Project Management
Browsing Pathways
Promoter analysis
Analyzing Dataset
    Changed Pathways
    GO Enrichment
    Functional classification
Searching
Adding New Platforms
Update Pathways and Genes
Contacts

Browsing Pathways

The "Browse" option on the menu bar allows users to observe or examine different biochemical pathways based on the selected expression dataset and/or metabolite dataset.

All Pathways

Selecting "All Pathways" shows all biochemical pathways for a particular condition in a folder tree format organized by the type of pathways. Options to open and close all folders are also provided. Pathways in black indicate no changes at all, while those in red indicate a change in gene expression/metabolite content.



Changed Pathways

Selecting "Changed Pathways" shows only the pathways that have any overall changes in gene expression and/or metabolite content, as well as the corresponding p values for each pathway indicating how significant the pathway changes.



Pathway details

In both "Browse All Pathways" and "Browse Changed Pathways" described above, each pathway is linked to its detail page. A pathway detail page includes a graphical visualization of the biochemical pathway with each molecule/metabolite highlighted with different colors to indicate the changes of gene expression or metabolite levels. Links to the list of all genes and metabolites in the pathway, as well as the link to the pathway in the original BioCyc database, are also provided in this page. Genes and metabolites in the pathway image are linked to pages containing their detail information.



Color legend

Plant MetGenMAP uses the following color legend to indicate differences of gene expression/metabolite level changes:
  1. Uploaded dataset contains p values.
     the ratio is greater than the up-regulation cutoff and the p value is significant
     the ratio is less than the down-regulation cutoff and the p value is significant
     the ratio does not meet either the up-regulation or down-regulation cutoff while the p value is significant
     the ratio is greater than the up-regulation cutoff while the p value is not significant
     the ratio is less than the down-regulation cutoff while the p value is not significant
     the ratio does not meet either the up-regulation or down-regulation cutoff and the p value is also not significant
     not measured or filtered out


  2. Uploaded dataset does not contain p values.
       the ratio is greater than the up-regulation cutoff
     the ratio is less than the down-regulation cutoff
     the ratio does not meet either the up-regulation or down-regulation cutoff
     not measured or filtered out



This site is supported by NSF IOS-0501778 and IOS-0923312