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Simple sequence repeat (SSR)
>UN54559
GGGACGCAGAACGAAAAAGAGAGAAAACACCGTGCGACGACGAAGAAGATTCTCTAATCTCTTCGTGTTAGGTTTAGCTCTTTTACAAAAGGGG
AATTAGCGAAGAGTGATACGATTTTCTTCTTCAGGCGGTGTTTGATTCGCTTATCGATTTGTTTAGTGTAAAACATATTATATAAATAGACTCT
AGAAATGATGATGATGATGGGTTTCGAGATCTCCGATGAGTTTCTAGGAACGTTCGTGCCGATTTTGGTGTATTGGGTGTATTCAGGGATGTAC
ATTTGCTTAGGATCTTTGGAGAGGTATCGATTGCATTCCAAGGTGGACGAGGACGAGAAGAATCTCGTCACCAAATCCGCCGTCGTCAAGGGCG
TTCTCCTTCAGCAGGCTCTCCAGGCCATCATCTCCGTTATCCTCTTCAAGATAACAGGAAGTGATGATGCAGATGCGTCTACGACGCAGCAATT
CTCCCTTCTACTCCTCGCTAGGCAGTTTATCATCGCCATGCTCGTTATCGACACTTGGCAATACTTCATCCATCGCTACATGCACATCAACAAG
TTCTTATACAAGCACATCCACTCTCAGCACCATCGCCTCATCGTTCCCTATTCATACGGAGCTTTATACAACCATCCACTAGAAAGCCTTCTCT
TGGACACCGTCAGTGGTGCTCTGTCTTTCCTCTTCTCCGGTATGTCCCCAAGAACCGCCATCTTTTTCTTCTCCTTCGCCACCATCAAGACGTT
GACGATCACTGTGGACTCTGGCTCCCCGGGAACCCTTTCACATCTTCTCACTAACACTCTGCTTACCATGATGTCATCATCAGCTCTATGG |
Primers for UN54559 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GCGGTGTTTGATTCGCTTAT |
60.103 |
20 |
CGAGATTCTTCTCGTCCTCG |
60.088 |
20 |
221 |
129 |
349 |
| 2 |
TTCTTCTTCAGGCGGTGTTT |
59.853 |
20 |
CGAGATTCTTCTCGTCCTCG |
60.088 |
20 |
232 |
118 |
349 |
| 3 |
TTTCTTCTTCAGGCGGTGTT |
59.853 |
20 |
CGAGATTCTTCTCGTCCTCG |
60.088 |
20 |
233 |
117 |
349 |
|
|
