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Simple sequence repeat (SSR)
>UN58133
GGATAGTTAAACAAACAGTTGCTAAGGTTGTTTTGAGAGGGAGAACGAAAGGGCGATCGATTAGGGTTTGTGTTCGTCGGAATATTCTATCTGA
TCGGAGGTACGAGCACGGTGGAGGCTGTGATAAAGTCAATCCAGGGACTATCATCGAGCTCCAGGGACTTATCCGCACTTCACGATGTTCTGAA
AGTAGCTAACGAGTCGCTCCGAGCCGACTCGGATCTTCAGCTTTCTACACTTGACCAGCTCGACCCCTCGAAGCATTCTCTCGGCTACCTCTAT
CTCCTTGTCCAATGATGTGGTTCTGCTGATTGCTCGGTTCATCAACTCTTGCGATGCTGTGCAGATTCGTTTAGCGAGCGAGAAATGTAATCTC
GTCTCTCTCTCTCTCTCTGTTGGTATCTTTAGGGTTATGATGTTGTTGTTTTGATGCTTGCAGTTGTTTCTCTTTGTAAGAGATTCAAAGACAG
AGTTTTGGAGCTCAGAGATCCTTTACGAGGGGTGGCGCCACTGTTGGAAGCTGTTCATAAGGTTCAGGTCTCCACCAAACGTTTGACTGCGTTG
CATCCGGATGTCTTCAGCTGTGTTTGCTAGCGAAGTGCTATAAAGCTGGTTTCTCCGTTCTTAGTGATGATATCTTGG |
Primers for UN58133 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TGTGGTTCTGCTGATTGCTC |
59.992 |
20 |
AAACGTTTGGTGGAGACCTG |
60.005 |
20 |
257 |
298 |
554 |
| 2 |
TTCATCAACTCTTGCGATGC |
59.955 |
20 |
AAACGTTTGGTGGAGACCTG |
60.005 |
20 |
235 |
320 |
554 |
| 3 |
CCAATGATGTGGTTCTGCTG |
60.112 |
20 |
AAACGTTTGGTGGAGACCTG |
60.005 |
20 |
264 |
291 |
554 |
|
|
