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Simple sequence repeat (SSR)
>UN61022
TTATATTTGTATTACTGAATGATAACGGTTACACAGTGAGTAATAAGGGACTCTCTCTCTCTCTCTCGCCCCCACATGAACCTAATGATCAAAC
AACTAATAACCTTCACTCTAAGTCAATACACCCTCACGACACACACACAATCTTCGTGATCCCAACATCCCATCTGCTCTGCTCAGATCCCTAT
TCTTGCAACTTCATTATTCTCTGAAGAAGATTCGTGGGCCAAATATGTACAAGACTCCTCTACCATTCTTGGCGCTTCATGCGTCGCGGCCCTT
ACTTTTGTAGTGAAAGATTATAGGAGGATGACACTCCTCGTATGTAATAAGAAAATCATCCCTGAAACCTAGAGCGAGAGGTTGAGACAGTACA
CTCACTCTGTTTTTATTCAGCAAACGGTCCGACCATAGCATATTGATGCAAGGTACAGATTTTTCGTCCATTTTTCCTTAACATGGGTGCTTGG
GAAAGCAGAAGTTCTGAGAGTCTCTTGGGTTTCATCGGTTATCTTTCGTTTGGGTATACTCAATATGAGAGCTGCTTGATCTGCTGTTGCAGCC
ATTGATGTTATCCTGAATCCACTCTCCCACCTTCGGTGAATTCCTTCACTTGGGTACAAAAAATCAAGCTCCACCACCTGTTCAGAATAGCCAG
AGTTTCTGGACATTACTACACCCCAACGACTCCCAGCAGTGGTCATGGAGGTTACATGAAACCCTTCTTTCCACTTTTTGTTTATCCATTTGAA
TGGGAATGAGTCGC |
Primers for UN61022 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TGAATGATAACGGTTACACAGTGAG |
60.333 |
25 |
AGCAGAGCAGATGGGATGTT |
59.834 |
20 |
162 |
16 |
177 |
| 2 |
TGAATGATAACGGTTACACAGTGAG |
60.333 |
25 |
CGCCAAGAATGGTAGAGGAG |
59.83 |
20 |
247 |
16 |
262 |
| 3 |
TGAATGATAACGGTTACACAGTGAG |
60.333 |
25 |
TGTACATATTTGGCCCACGA |
59.809 |
20 |
224 |
16 |
239 |
|
|
