 |
Simple sequence repeat (SSR)
>UN61999
GGACAATCATAATGTTTTCGAATTTCCTCGAAAGCTTATACGATGGCATCGGAGACGATGAAGCCGACGACGACGACGAAGAGAACAGCCCGAA
AACTGCAACGGAGAGACACGTTCCCTAGAGAAACACCGATCGTTTGCCTCCTCCGATGTCGCCGGAAGAGGAGGAGGCCCAAGCGCGCGGGGTG
AAAGACGACCTGTCGGAACTCAGCCATACCCTCTCGCGTAAATTTCGTGGCGTCGCTAACTTTCTCGCACCGTTACCGGAGAGATCTTCGTCAG
ATCTGTCGAATCCCAGATTGAGCCAGTCGCGTTTTTCGGATCCAGAATTGAATAAAACCTCGTCTTCCGATCCAAAGCTGCATCGATCGTCCTC
TTCCGATCCCAGGCTGAATCAATCGGCGTCGTATTCAGATCGGCCTGGATCGTGTGTGGGAATTGACACGCGAGAGACAGAGATTAGAGCTAAG
AGCTGGAATTCGGATAATATGGAAGATAAAAGATTAGAAGATTTACACAACGATCTGGAGGAGGAGGAAGAGGAAGAAGAAGAAGAGGAAACGG
ATGAAGAAGAAGAAGAAGAAGAAGAGATGGATGCGGTTGCTCTGACGGACGAAGTGCTAGCATTTGCGAGGAACATAGCAATGCATCCTGAAAC
TTGGTTAGACTTTCCTCTCGACCCCGACGAAGACCTTGATGATTGGGAATGTCTGATGCACAAAGAGGCATGCTTTAGCTATAGAACGTCTTGC
TCCGAGGTTAGCCGCCCTCAGAATTGACTCTGTCCATGCCATATGACTGT |
Primers for UN61999 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
ACACAACGATCTGGAGGAGG |
60.112 |
20 |
TGCCTCTTTGTGCATCAGAC |
59.992 |
20 |
214 |
515 |
728 |
| 2 |
ACACAACGATCTGGAGGAGG |
60.112 |
20 |
ATCATCAAGGTCTTCGTCGG |
60.073 |
20 |
187 |
515 |
701 |
| 3 |
ACACAACGATCTGGAGGAGG |
60.112 |
20 |
AATGCTAGCACTTCGTCCGT |
59.904 |
20 |
114 |
515 |
628 |
|
|
