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Simple sequence repeat (SSR)
>UN05500
GGGAGACAGAGAGAGAGAGAGACCACTTCGCTCCTCCGGTGATAGGTTTTTATTCCGTGTGTGACTGTTTGTTTGTAATTCACTCAAAACAATG
GGAGACTCAGAAAACGCGCAGCCACCTTCTAAAAAGAGAGGTGCTTTGAAGCAGCTCTCTCGTGACAATCCAGGTCTCGATGATGATGATGATG
ATGTTGATACATCTGAATTGGAGAGTGGGACTTTCAAGAAAGCCAGTGATGAGGTTTTGGCTAGTAGGAGAATCGTCAGAGTCAAGCGCAAAGA
GCCTTCCTCTGCTCCACCACCATCCGCTGCAGCAACGACCAATCCCTTTGCTGGGATTCAACTGCTCCCTACTGCACATGCTTCAACCGCTAAT
CCAACTCTGGCTGAGTCTAAGTTGGCTCCTGCTGAAGCCGTAGTTGAAGACAATCAGAAGGAAACTGATACCGAAGAGGGCGGTGATGAAGTTG
AGAGTAAGAAGAAGGTTGATGATGTCAAAGACTCTGCTGAGGAAGAGACTAAAGATGATAATGAGCGTGGAAAGACATCTGATGACCAGACTAC
TCATGATGATGATGTTAAAGAATCTGCTGAGGAAGCGACAAAAGATGATAATGAGCGTGGAAAGACATCTGATGATGATGTAGACAA |
Primers for UN05500 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GCTCCTCCGGTGATAGGTTT |
60.464 |
20 |
GGAGCAGAGGAAGGCTCTTT |
60.096 |
20 |
268 |
30 |
297 |
| 2 |
GCTTTGAAGCAGCTCTCTCG |
60.559 |
20 |
TGTGCAGTAGGGAGCAGTTG |
60.049 |
20 |
224 |
137 |
360 |
| 3 |
TTTGAAGCAGCTCTCTCGTG |
59.465 |
20 |
GGAGCAGAGGAAGGCTCTTT |
60.096 |
20 |
159 |
139 |
297 |
|
|
