 |
Simple sequence repeat (SSR)
>UN65016
AGATAGACTTTTCCATAAGAGAAAAATGATCGAGTTTTGCAATATGAAACAAACAGAAAATACTTAAACCAAAACACAAACTTAGTACTACATG
TTTACAGGGACAAACCCAAACGGAAGACATCATACACAAAACAAACATGGAACATAGTAGCCCCCACAAAAAAAGAAACGTGCACTTGTCCGTC
CTCTGAGCTAACGAGTGCCCTGTTTCTTGTTTCTGCCAAAAGTTCTGGTAAACCACTCCTGCTTGTATCCAACCACTACGTATCCGATGGTAAA
TCCCAATACAATTCCAGGTGCAAAGCCTAACCCTGCTGCAATCCAACTCAAAGTTTCCTCCTCTGCTTCTTCTTCTTCTTCTGATGAGACTGGC
ACTTCAGGTTCTGGTGGTGTTGGTTCTCCACAAATATCCTTAAGGGAAGGACCATAAAGTCCGGGGTTTCCCTCGTATGAAGAGCAGTTTTGTC
GCTGGAACTGTGTACCCTGTGGTACTGAACCTTGGAGTTGATTATGTGAAACATTTATCCACGCAAGAGAAGAGAGGTCTCCGAGCTTCGGAGG
GATCTCACCAGAAAGCTTGTTTTGGGATAGGTCCAGTGATTCCAGCGCAGTGATGTTTGCCAAAGACTGTGGGATGTGTCCTGTGAAAGCATTT
CTTGACAGATTGAGAACATGGAGTTCCTTCAACAGACCGATGGAT |
Primers for UN65016 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GTCCGTCCTCTGAGCTAACG |
60.012 |
20 |
TTTGTGGAGAACCAACACCA |
59.976 |
20 |
229 |
182 |
410 |
| 2 |
CTGCTGCAATCCAACTCAAA |
59.988 |
20 |
GAAACCCCGGACTTTATGGT |
60.053 |
20 |
132 |
315 |
446 |
| 3 |
TGCTGCAATCCAACTCAAAG |
59.988 |
20 |
GAAACCCCGGACTTTATGGT |
60.053 |
20 |
131 |
316 |
446 |
|
|
