Simple sequence repeat (SSR)
>UN65096
TTTTTCTTCTTCTCTCTCTCACATCAGAACTATTTAACCCTTCCCCCTGTCAAGTTTGAGCAACATTTTTCCTCTGAAAGATGGGTTTCTCGAA
CGCTGGAATTGGTCTCTCCGATCAAAACAATCTCTGCGAAACGGAACTAGGGTTTTCAGACCAAAGCGTTCAAGAACTCCGGCCAGATCAAGAT
CTTGAAACTAAAGTCGGAGAGATACCTAATACTCAACGCGTTGGAGAAAAGGTAAAGAAAGAAGAAGAAGAAGATTTATGTAAGACTCCGACAC
GCCTCGACCAGATTCTCCCGAATATTATAAATATTTGTCCTCCGGCGCCAAGGAAGCCTAAGAGAGTCCCATCGAGAAGTTTGAAGGCTCGAGA
TTCTTATAGAAGCAGGAGAAGGATCATTTTGAATGTTTCTAGAGAGATCGATTGTATGTTTCACTCAGCATCTCTAGGTAATCAAGTTAAGAAA
GCCAGACGTATTTGATGTTTCATATGTATATGTATAACGTAGCTACATGTAACCAGATTGTAATAAACCACGAGTTATGAAATGTAGTGGGATG
CCATTTCAGTTTTGG |
Primers for UN65096 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CTCTGCGAAACGGAACTAGG |
60.008 |
20 |
TATTCGGGAGAATCTGGTCG |
60.029 |
20 |
181 |
126 |
306 |
| 2 |
CTCTGCGAAACGGAACTAGG |
60.008 |
20 |
GAGGCGTGTCGGAGTCTTAC |
59.874 |
20 |
162 |
126 |
287 |
| 3 |
GGAATTGGTCTCTCCGATCA |
60.011 |
20 |
GAGGCGTGTCGGAGTCTTAC |
59.874 |
20 |
189 |
99 |
287 |
|
|
