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Simple sequence repeat (SSR)

>UN65184
GCAACCGTCACCTAACGGAAGGAAGGTGATGCCATCTCCTAGTGGAAGGAGACTGTCGATTTCGCCGGTAAGGAGGCTTTCAAGTGGTTTGAAA
ATGTCTCCCATGGTTGGAGATTGTTCGGGGAAGAAGAAGATGGCGGCAATTGCTGCGGGAATCTCTAAAGTGTCTGAGGCTCTTGTCGGATCTT
CAGCTAAGAATGGTAACCGAAAGAATTGGGATGAACCTGATGAAAGTGTTCAATCTGAGCACAAGGAGAAGAAGAAGACTGATCTTCAGGGGAT
TTTGCGTACTCAGGCTGCTATGACGAGACGTCTTAGTGATGCAAATAGGCGAAAGAGTGATGCTTCAGCATGTGAAGAAAAAGCCAAGTCTTGT
TCATCTGCGAATAGTTTAGTGGAAGAAGAAGAAGAAAAAGTCTCAGCTTTTGAAGGACTTGGGATCACTTATCATGATAGGAAATGGACAGATG
GTAGTGTTCCACTGGATAGCATCTCAGGGGATCTCGCTAGACTTGCAAAGGAAGCTTTGCAAAGAAGAAACTTTGCTGCTAAAGCTGCAGCTCG
TGCATTGGAGGAGGCTAATGCCAATGAGTGTATCATTAGATGTTTAAGTAAATTCGCTGAGCTTTCTTCAGCCTCCAAA

Primers for UN65184 SSR

Primer pairForward primer (5'-3') Reverse primer (5'-3')Product
SequenceTemperatureSizeSequenceTemperature SizeSizeStartEnd
1 TGATCTTCAGGGGATTTTGC 60.014 20 CCCAAGTCCTTCAAAAGCTG 59.846 20 172 268 439
2 TGCGTACTCAGGCTGCTATG 60.18 20 CCCAAGTCCTTCAAAAGCTG 59.846 20 155 285 439
3 TGGTAACCGAAAGAATTGGG 59.795 20 CCCAAGTCCTTCAAAAGCTG 59.846 20 241 199 439