 |
Simple sequence repeat (SSR)
>UN00558
GGAGCTCGCTCTCACAGTAGCCCTAATAAAAAGAAAGAGCCCCCCCATATTCGATAACAACTTTGCTTCTCTTCCTTTCTCGTTTCTGATCCGT
AACCCTAAATTATCCACATCCGCCGCATCATCAGATCCTTCTCCTTCCATCCGTTCCGTCGACACTCCCAGCTGAAATCGCCGAATTCCGTTTC
CGAGTTCTCATAAAAACCGAACCTTTTTAGCTGTCACTACCTGAATCGGAGGTTGTTGTTGTTGTTGTTTGAGATAAGATGGTGGGTGGTGGAA
ACAGGAGAGACGAAGGATCGATGCCGATTCAGAACACCAACTTGTTCGCCGCTTTGGATACTCGGAGTAAGAAGAAGAAGTCGTCGTCCGACAA
GAGCAAAGGACGTCAAGATCCGGTGAAGGAGGAGCCTGAGCCGCAGGTCTTCTGGTCTCCTACGCCTCTCAAAGCCAAGGCTTGGGCGGATATC
GACAGCGACGATGAAGATGATGACTATTTCGCCACCACTGCTCCTCCCAAGGCCTTGTGGAGTGCCTCTGAAGCTTCCCGTTCCGATGCGAAAG
AGGTTCAAGTTGAGGAAAGTGAGAGTGAAGAAGACATTCTTGACGAAGGTGATGATGATGATGATGATTTGGAGGAGGAGCATGAGACGCAAGT
TCATCCAGAAGCAGAGCCTGAAGTGAAGAAGGCTCCTGAAGTTCCTGCACCACCCAAGGAGCCAGAGAGGCAGCTTTCCAAGAAAGAGCTGAAA
CAGAAGGAACTTGCTGAGTTTGAGCTTTGTTAGCAGATTTTGGAATTGCACCAAGAGATATGCG |
Primers for UN00558 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TATCGACAGCGACGATGAAG |
59.972 |
20 |
CTGGATGAACTTGCGTCTCA |
59.984 |
20 |
200 |
467 |
666 |
| 2 |
TATCGACAGCGACGATGAAG |
59.972 |
20 |
AACTTGCGTCTCATGCTCCT |
60.02 |
20 |
193 |
467 |
659 |
| 3 |
GACAGCGACGATGAAGATGA |
59.95 |
20 |
CTGGATGAACTTGCGTCTCA |
59.984 |
20 |
196 |
471 |
666 |
|
|
