 |
Simple sequence repeat (SSR)
>UN66479
ACAGGAAAATATTTTTAACTTTTTATTTGATAGAAAAAGAAACAGAGTGTTCAACCCTTATATGTCCATCAATCAAAAGAACACAAATGAAGAA
GTGAAGGAGAAAAGAATGGTAAAAGAGTATTTATTACTAAAGCCAACAACAGGAAGCTCAGCCTCAAATGGGTTTGGAGGAAGAAGAAGAAGAA
GATGATTTCCTGTTTTTTGCAAAGTCAACCAAGTCTTTGAAAAGGATATCCTCTGGTTTATCATCTTTCTTAGGTGGTGGTGTAGCAGTAGAGG
CGGTTGGATTCAGAGAGAGATTCCTGGATTGCGCCAAGAGATCATCGTAAGAGGAATCAGAGCCACTGCTTGAGTTTTGAGGAACATTATTATT
ATGCTTGGTTCTAACAGACATTGGTGGTGGAACAACCCTGGTTCCTTCAGTATCCCATGGAGCACGAGGCAGTTTCTCAGTTTGGTCGTAAACT
GGTTTACTGAACAAAGGTTGCTCAGGTGATTTGCTTCTAGGAACTGGCTCATCGAAAATCGGAGCAGAAGAGTCGAGGTCTGATGAATGATGGG
AAGGCTTTACACTCTCTGAGGTTCCTTGAGGTTTGTAGACATCACCACTGAGGAAGTCCATAGCATTAGATTCAACGTGTACTGGCCTCATTGT
AGGTGGCGGAGGAGGAGGAAGAACAGGGTTGAAACTCACTCGTGCGCTCTCTCTTTTCGACCTGTGAGATAGCTGAGCAAAATCATCATCTGAC
TCATCATCATCATCGTCATGGTTGATGCTAACAAGCGGTATAGAGCGGGAGCTGTTGCAGTAGGAACAGAGTTTCTTTCGCCTTATCATCATGT
TGCTGAAAACACGCTGCAGTTGTCGTTTATGC |
Primers for UN66479 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
ACAGGAAGCTCAGCCTCAAA |
60.134 |
20 |
CTCTCTCTGAATCCAACCGC |
59.95 |
20 |
159 |
143 |
301 |
| 2 |
AACAGGAAGCTCAGCCTCAA |
60.134 |
20 |
CTCTCTCTGAATCCAACCGC |
59.95 |
20 |
160 |
142 |
301 |
| 3 |
ACAGGAAGCTCAGCCTCAAA |
60.134 |
20 |
CCAACCGCCTCTACTGCTAC |
59.898 |
20 |
147 |
143 |
289 |
|
|
