 |
Simple sequence repeat (SSR)
>UN66650
TTTTTTGTTTTCCTCCTATATCAAACTTCAAATCTCTTTCTTCTCTCTGCTTCATCGTCACAAGTAACCAACCATCATGGACACACTCGTCGCT
CAGCTGCAGAGACAGTTTCGTGACTACACCATCTCTCTCTATCACCAGGGGTTTCTGGATGATCAGTTCACTGAATTGAAAAAGCTGCAAGATG
AATGTAGCCCTGATTTTGTGGCTGAGGTTGTTTCTCTCTTCTTTGAAGACTGTGAGAAGCTTATCGGTAACATGGCTAGAGCTCTAGATCAGAC
AGGAAACGTAGATTTCAATCTGGTAGGTTCTAGTGTTCATCAACTCAAAGGTAGTAGCTCAAGTGTTGGTGTCAAGAGGGTCAAAGGTCTTTGT
ATTACCTTCAAGGAGTGTTGTGACTCTCAGAACTTTGAAGGGTGTGTGAGATGTTTGCAGCAGGTGGATATTGAGTACAAGTCATTGAAGGCAA
AGCTTCAAGATTTGTTCAATCAGATCGTCCAAGCTGGTGGTAGAATCCCTCAGGTTGATATATAAACTACTTGTGGACGAACATATCGATGATA
ATGATGATGATGATGATGATGATGATTTGCATCCTCAGATTTTATTGTTTAGTTAAAACTTTTTACAAATGTGTGGGAAAGACTGGTGATTGTT
GTTGTTAGATCAGTACCGTGTAACTTTATCAATCGCTTTGGTTTGACG |
Primers for UN66650 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GCTGGTGGTAGAATCCCTCA |
60.073 |
20 |
TCACCAGTCTTTCCCACACA |
60.129 |
20 |
151 |
503 |
653 |
| 2 |
ATCGTCCAAGCTGGTGGTAG |
60.134 |
20 |
TCACCAGTCTTTCCCACACA |
60.129 |
20 |
160 |
494 |
653 |
| 3 |
TGCAGCAGGTGGATATTGAG |
59.823 |
20 |
TCACCAGTCTTTCCCACACA |
60.129 |
20 |
222 |
432 |
653 |
|
|
