Simple sequence repeat (SSR)
>UN67000
GGACTCCAAAAACGATCGATCAATCTGAATCTTCTCATTTTCCCCCTTTCTCCGTTTCATAGCTAGCTAGGGTTTCGAGATGGGTCAGATCCAG
TACTCTGACAAATACTTCGACGATACTTTCGAGTACAGGCACGTCGTGCTTCCTCCTGAAGTCGCTAAGCTTCTCCCGAAGAATCGTCTTCTCT
CCGAAAATGAGTGGCGTGCGATTGGGGTGCAGCAGAGCCGTGGATGGGTCCATTACGCCATTCATCGTCCAGAGCCACACATCATGCTGTTCAG
GAGGACTCTCAATTACCAGCAGCAGCAGCAGGAGAACCAAGCTCAAAACCTGCTCGCTAAGTGAATCTGAGTTGTGCTGCGCTACAAACATTGG
TTCTTGTTAGCTATTATGTTTCTTGTAAACATGGGTTTGTGAACCCCACAACCTCTAATAACTTTGTGTTCATGTTATCATCCTTGGTTGGCGA
AAAACATGCGAGTGAATGAG |
Primers for UN67000 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GGTCCATTACGCCATTCATC |
60.163 |
20 |
TTTGTAGCGCAGCACAACTC |
60.203 |
20 |
136 |
235 |
370 |
| 2 |
GGTCCATTACGCCATTCATC |
60.163 |
20 |
TAGCGCAGCACAACTCAGAT |
59.77 |
20 |
132 |
235 |
366 |
| 3 |
GGTCCATTACGCCATTCATC |
60.163 |
20 |
GAACCAATGTTTGTAGCGCA |
59.735 |
20 |
145 |
235 |
379 |
|
|
