Simple sequence repeat (SSR)
>UN67456
AAGTTAAAGTTGTTTGATGAGGAAGAGTAATAAGATTGTCTAAAAAGTACACAAATGCATGAACAAAAAAAAAAAAAAAAGAGGAAAGTAACGT
TTATAAACCCTAAAGTTCCTCAAGACTCAACTTTAAAACTTGTAAACTCTTTCAAAGCGCTAAAACAAGCCGAGTCTCTAAAAACTGTAAGGAG
CAAATAAGTTTTGTTCTTAGTTCTTCTTGCTCTTTGATTTCCCAGACAATGTCTTGGCCAAATGAGTTCCCTCGAGCCACTCGTCCATTGATGC
GTCGGTAGACCTGGTTCCTACTTCCACCTTGGACACCTTTTTGGTTTTCTTAGTGAGACGCTGCACTTGGCTGTATGCCCATAGACCGAGGAGG
AGGAGGAGAGCAATCCCAAGTGTTACGAGGAAGACAGACTCACCGCTGAAAAGACCTCCAGATTCAACAACCTCAATGGTCCCATTGTAAAA |
Primers for UN67456 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TAGTGAGACGCTGCACTTGG |
60.199 |
20 |
GGTCTTTTCAGCGGTGAGTC |
59.851 |
20 |
100 |
333 |
432 |
| 2 |
CGCTAAAACAAGCCGAGTCT |
59.654 |
20 |
GCGGTGAGTCTGTCTTCCTC |
59.993 |
20 |
271 |
152 |
422 |
| 3 |
CACCTTGGACACCTTTTTGG |
60.38 |
20 |
GCGGTGAGTCTGTCTTCCTC |
59.993 |
20 |
116 |
307 |
422 |
|
|
