 |
Simple sequence repeat (SSR)
>UN05822
CGAAGAAAAGACAAGAGTCTTTAATGATGACAAAAAAAAGTAGCATTCTTAATTCCCAGCACATGAAAGGAGGATTGGAATACCAAATAGCTAA
CAAAGTTATAAAACAAAGACGATAAGCATTGTAAAGACGCAAAATCTCAGTGCCCATCGTTGTGCTTCACCTCAAACAGACCGTCTGGACCCCA
AGGAAACTCCTTGTTACGGATGTGCATATAAGGATACGCAGGAGGATCGTCGCCGTGAAGATGACCCTTGGACAAAACATAGGCGGCAAGAAGA
GTGCAACTAAAAATGCCCCCGTATGTTATGCTCTCCCACTTTCTAGCTTCGAAAGCATCGTCAAGGCCGGCAGAAGATGAAAATCTCCGCTTTG
GAGCAAGGGATGTCCTCGGAGTTGAGCGGGTAGCTGCTCGGGATAGAGCTGAACGTATTATCGCCGTCGCCATTCTCCTGATCTCTCTCTCTCT
CTCTCTCTCTCTCTCTCTCTCTCGTTCCCCCCGGCAAAATCTGCAAAAAAAAGAAAAAAAAATCCATCGGATCGACTGTCGAGTCCACGGCTCT
TCTTCGTTCGTTTTTTTTGTTTTTCCTCCCCCTCCTCCTCATCGGAGCTGATGTTTTTTTTTTGAATTCTGATCAGATCTAAGTCCTAAGAGAG
ATTATTATTA |
Primers for UN05822 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GCTGAACGTATTATCGCCGT |
60.124 |
20 |
CAGTCGATCCGATGGATTTT |
59.894 |
20 |
125 |
424 |
548 |
| 2 |
GCTGAACGTATTATCGCCGT |
60.124 |
20 |
AGGGGGAGGAAAAACAAAAA |
59.787 |
20 |
174 |
424 |
597 |
| 3 |
GCTGAACGTATTATCGCCGT |
60.124 |
20 |
AAACATCAGCTCCGATGAGG |
60.218 |
20 |
197 |
424 |
620 |
|
|
