Simple sequence repeat (SSR)
>UN71000
GGAGGGTTTTGAAGGGACAAGGTAGGTTTATCTCTTAACCACAATCATGGAGTCTCTGGGAGGATTGCAGCTCCATCACCAGCCATTTCACCTT
TCCTTCACTCGCACCTCTTCACCGTTCCCGAAACACCCAACATCTTCCCCTTTCAGACCATTAATCCGACCAAACTCCTCTTTCAAACACGTTT
CAATCAAAGCTTCCTCCTCCAAATCACAAAACCCTATCACCCCTCCCCTAAAGTCAACACCTTTCGGTCTCCTCAAATCCACATGCATCACTCT
AACAACCGCCGCCGCCCTCCTCTTCATCAACCTCCAGCTCAAAACTCCAGCGATTGCAGCTCCCGTCGCGCCACCACCACCGTCACGGAAAGAC
AAGACACGTCACTTGGAAGAGAGAGAGAGCGCTCGAGAGCATCTAGCACACACCTCTGACGTCGACTCTCTCGTCCTGATGAGTAAGATCAGAT
CTCGTAGCTCGAGAGCATAGAGTAGTCGACGTTGATCAACTAGACCCGAGAGACAGACGCTGTCTAGAGCTAC |
Primers for UN71000 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CCTCCAGCTCAAAACTCCAG |
59.982 |
20 |
GTCGACGTCAGAGGTGTGTG |
60.368 |
20 |
130 |
313 |
442 |
| 2 |
TTCGGTCTCCTCAAATCCAC |
60.05 |
20 |
GTCGACGTCAGAGGTGTGTG |
60.368 |
20 |
192 |
251 |
442 |
| 3 |
CGGTCTCCTCAAATCCACAT |
59.927 |
20 |
GTCGACGTCAGAGGTGTGTG |
60.368 |
20 |
190 |
253 |
442 |
|
|
