 |
Simple sequence repeat (SSR)
>UN71227
ACTAGATTAACTTTTTACCGCCCCGAATGTTACGAATTTACAATGAAGACCCATTTGATGTTGGATTAACTCAACTGTATTCAAACTTTCTTAA
GTGTTAATTTTAGAAGCAGAAAAAACATATGAAACTTTCTGAGGTCGTTTGACCAATACGGTTGCAAGTACAATTAATGTGTTATTTATTATCA
TTATTAGTGGTGGTGGTGAAACTTGTTGTCGGAGGAGACTTGGAGATTATCAATGCCTTTAATAGCTGATTCGTAGTGTCTTCTTGAGGAAGAA
GACCTCTTCCCAGCCTCCACTGGGGAGTTCCTAGAGATCACTCCTCCTCCTCCTCGGCTTACGTCACCTGTACGGTTTCTCTGAAACTCTTCCC
CGCTCAGAAGATTGTCTCGGCTGCTACTCACTGCAGCTACTCTCCTCGACGATCCTGCTGATTGTGCAAACATCGAGGAGCTCGGCATCATGGA
GTCTCTGTTAAATGATGATGTCTGATGGTTGGAAAGATTCCCTGAGTGGTCAAGAGTGCCTGAAGCTCTCCGTCGAGATGATTCCATATGCGAG
AAACCAACCGGTCTCCCTTCTTCCTCGCCTGTGTATCTATCAATGTTTGAAACGCCAGGTGGCAATGCGGCGCTAGTACCAACCGCAGGGTTGA
GATTACGAGTCGGAGGAGCGGTTAGTTGTGATTGTTGGTACTTCAAGATGGTCCAGTCAAAACATAATCAAATTGAAATCCTTCACGGATAAAG
AGGTCTCTGAATAT |
Primers for UN71227 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TGGTGAAACTTGTTGTCGGA |
60.128 |
20 |
GAGACAATCTTCTGAGCGGG |
59.95 |
20 |
193 |
202 |
394 |
| 2 |
AAGAAGACCTCTTCCCAGCC |
59.817 |
20 |
CAGGCACTCTTGACCACTCA |
60.022 |
20 |
255 |
278 |
532 |
| 3 |
AAGAAGACCTCTTCCCAGCC |
59.817 |
20 |
ATGGAATCATCTCGACGGAG |
60.034 |
20 |
280 |
278 |
557 |
|
|
