Simple sequence repeat (SSR)
>UN71321
CGGGAAGCATTACATAATAGATACAGTGGCAACACGATTTTACAGCATTTTTTTTAAGAAGAACAAACGACTTTCAAAGAAAATGGAATCATAA
TCGTGATACTAAAGTGGGTTGCTCCAAACTCATCTTAGGAACCTTTTTTTTGTTGTTGTGGGTACTTTCAAGAAAGACAATAAAAAACATAAAA
TATCATTGCTTTAGATCTCTAAGCAATGTGACTTTTTCTTTGGGGGGGAAGCAAAGCAAAATCCATCTTCGTGTCGTTCAGGAGTCCACAGCTG
GAGATTGCATGCAGCAAAGCCATTTTTGGGCTGGGGTTTACTAGGATCACCTCCTCCTCCTCCTCCGGACTTAGCATGACCATCAGTGGATTTA
CCAGGTGAACCGGGTTTGCCACCGGTCTTCTTCTCATCCCTAGCTTTGTTGAATATGACTGTGAAACCTTCAGCTGATGCTGGATCGTTCACAT
CCCATTCACCAAACTTGGGCAAGGACGACCTTTGTCCGACATAGGGGTGTGCAGAAAGGAGGAAGATTTAATCCCAGAGAATTAATGGAAAGAA
ACGATTCGAAAGAAAAA |
Primers for UN71321 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
ATTTTTGGGCTGGGGTTTAC |
60.055 |
20 |
ATGTGAACGATCCAGCATCA |
60.08 |
20 |
167 |
304 |
470 |
| 2 |
TGTGACTTTTTCTTTGGGGG |
59.942 |
20 |
ATGTGAACGATCCAGCATCA |
60.08 |
20 |
256 |
215 |
470 |
| 3 |
ATTTTTGGGCTGGGGTTTAC |
60.055 |
20 |
CTTCCTCCTTTCTGCACACC |
59.844 |
20 |
232 |
304 |
535 |
|
|
