 |
Simple sequence repeat (SSR)
>UN73118
TTTTTTATTTTCGTGTTTCTTTTTTTCACTTCTTTTTTTGTTCTTCTTCTCGTTGTTTGTTCGTCTCTTTTGTCTTAACGAAGAACAGGGTGAT
TTATGTCGGCTGGGTTTGAATCAAAAGCTGTGATTTTGTCTCTGCTTAACAACAACATCTCTTGTCTGATTTCATCATCATCTTCAACTAAATA
TTGTCTGGCAAACGATCTTCACTTCTGCTCTAATAAGCAGAGGCTTGTTCTCGAGAGTCCTTCTCTCTCCATGCGCTTAAGACCCAAAAGGACT
TGTTCTGGCGTTGAAGTCTTCGGTGGTTTTCACATAAAACAGTTCACTGTTCGCTGAGATTTTTTTTATTACAAAACAAGTTTTGATCTGAAGA
GTGTTTTGCCTTTTTTTTTATTTTTCTGGGTGTTTTTTTTTATCAAAAAATGGTAGGGATTATAAGTAAACAACTGTCTTTGAGTGATATGAAG
AAACCAAAGAGATGCAATCCTCAAGAAACCCAACAGCCTTTGAGAAAAGTCAGGATCTTGGTCAACGATCCTTACGCAACCGATGATGATTCAT
CTAGCGACGAGGGAGAGGATACCAAGACGAGACGCATCGTCCGCGTTGTTAACTTCCCACCTCTGGAGTCTGTTGCTCCTCCTCCTCCTCGTAC
CGAGTCTGCTTCTGTGAGTTCTTCACAGGACAGCACCAAAACAATCAGCAAGAAAGCAGCAGCAGCAGCTTCTCGTCCTCGGCGTTCTAGAAGC
CTGTTGGTGTCA |
Primers for UN73118 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GCAATCCTCAAGAAACCCAA |
60.051 |
20 |
GCTTCTAGAACGCCGAGGAC |
61.42 |
20 |
269 |
484 |
752 |
| 2 |
TAGCGACGAGGGAGAGGATA |
59.933 |
20 |
GCTTCTAGAACGCCGAGGAC |
61.42 |
20 |
187 |
566 |
752 |
| 3 |
CATCTAGCGACGAGGGAGAG |
60.111 |
20 |
GCTTCTAGAACGCCGAGGAC |
61.42 |
20 |
191 |
562 |
752 |
|
|
