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Simple sequence repeat (SSR)
>UN74500
GGGAGACTCAGTACCATTTCTTCTTCCACCAATTTCTCTGACGCGCTTTCTTCTCAGCTCTCTCATCTTCACGTCCGGCGCATTTCGTGCGAAG
AGCTAAAGGTTTTTTTTTTTTTTGGAGCTCATGGACAGCTTCTGGCAATTGGGTGATGAGCTACGAGGCCAGACACAGTCACGAGCATCAGAGG
ATCACAAATGGTCCACCGTCGCAACCAAGCTCGCTGAGCAGACTAGGATGAAAAGCGAAAGGTCGAACAACCTCGATCTCTCCAAAGCTGGCTA
CTCCGAGTTCAGACCATCAGACAAGCTTAGTTTCCACGACAACAACAACAACAACCTTAGCTTCAACATGTTTAACAACAGCAATGTTTACAAC
AACATGAATGCTTCTGTTTTCCAGAAGAACGACTTCTTTAAAAGCGGAGGAGGCAATATGAAAGTTAACAAGTATAGTGGCAATGTTGTTGCTA
ACAAAGAGCTGAGTAATAACAGCAACAAACACAACAACAGCAATTATAGTGCTGTTGACAAGAGGTTTAAGACTCTGCCTGCGTCCGAGACGCT
TCCGAGGAGCGAAGTTCTTGGCGGGTACATCTTTGTTTGTAACAACGATACTATGGAGGAGGACTTGAAACGCCACCTGTTTGGGTTACCTCCA
AGATACAGGGACTCTGTGAGGGCATAACACCTGGATTGCCTCTGTTTCTTTACAATACACCACTCACCAGCT |
Primers for UN74500 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CGAGCATCAGAGGATCACAA |
59.942 |
20 |
ATATTGCCTCCTCCGCTTTT |
60.061 |
20 |
260 |
176 |
435 |
| 2 |
AGCTCGCTGAGCAGACTAGG |
60.058 |
20 |
ATATTGCCTCCTCCGCTTTT |
60.061 |
20 |
220 |
216 |
435 |
| 3 |
CCAGACACAGTCACGAGCAT |
59.897 |
20 |
ATATTGCCTCCTCCGCTTTT |
60.061 |
20 |
273 |
163 |
435 |
|
|
