Simple sequence repeat (SSR)
>UN75114
ACGTCTAAAATAATTAAATAAATAAACATGTTTTATGTATGGGTGTCATTTGTAACAAAGTAAAAAAGAAAAGAAAAAGGATATAGGCTTGTCT
CTTATCTGCAGCAGCAGCAGCAGCAGTTGGAGTTTTAAAGCTCGACGATGCAGCAGCTCTTTTCCTGGTTGAGACGGCATATTCTGGCGATGAT
TCTCTCTGTCTTCAGTGTCTTTTACGGACTTTTTGCCCTGTGCTGAATCATATACAAATTAGTGACAGTTCTTTGTCCTGTGTAATTTCTAAAA
GCAAGAGAAGAAGAGCAACAGACCAATAATATTCTTCTGCATTGTAAGAAGACGTGTCTCGAGTCTAGACAAAACTGTTGTACGCTCAAAACCT
TGCTTATCATGAGCAGGGTCAACGAAATTAGCTTCCAAAACACCTATCTATGACCGCACGACCTTGACTTCCTGCTGCATTCCATATAAGGCGA
TGCTTGTGGTAAAAATATTGCAGCCATAATGGTCACAACAGCAGACATATTTGAGAAAT |
Primers for UN75114 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TGTTTTATGTATGGGTGTCATTTG |
58.721 |
24 |
AATATGCCGTCTCAACCAGG |
59.955 |
20 |
149 |
29 |
177 |
| 2 |
TGTTTTATGTATGGGTGTCATTTG |
58.721 |
24 |
CAGGGCAAAAAGTCCGTAAA |
60.103 |
20 |
200 |
29 |
228 |
| 3 |
TGTTTTATGTATGGGTGTCATTTG |
58.721 |
24 |
AATCATCGCCAGAATATGCC |
59.891 |
20 |
161 |
29 |
189 |
|
|
