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Simple sequence repeat (SSR)
>UN77400
AACACCTTTACGTAAAGTTGATACCTTTATTATCTATCAAACAAGAGCTAAGTTCAAAGAACAAGATCCAGAACAAATCGAAGAAACTGGAATT
GTTCTTCATTACATAAGTAGATATGAAAACAAAAGAAAGCTACTTCCTGTTTTCTTCTTCTATCTATGGGTACTCGAGGACTCTTTTTAAACTG
GTGACAAAAGAGCATACTCGTGTGGACATGTACGTAACGAAATTGTTTGTAAAACTACTAAGTAGCTTTGATCGAACGATCTGAGCTTTTTTTG
AAAACCCTAAAATCGATCACTTTATTCTTCCATTTGCCCATGTGCGTGCAGACCTGTGTGGAGCGTGCCAAAGAGTTTGTTTCAACGTTATTTT
TCTTTGGGCTTTCTTTAAGATTCTCTTACAGTCTTGGGCTTGAAACAAAGACAGTGAGACTGCTTCTTCTGTTTCTTTGTCTCCCTCTTTCCCT
TCTTCCTGCTCTGATTCTGATCTCGAGTTTTAGCATTACCCCTGAAACAATTCAAGAAACTGAATTTCTTACGGTGAACTGGTTTCTCTTCAGT
CTCCCCTGAACTCTGAGTTATCGGTCTTTCGCTTGTTTTCTCTGATGAAGCAACCTCCGTCCTTGTTTCTATAGGTTTAGGAGGAGGAGGAGGA
GGTATTGTTTTCTCCAAAGACAATCTAAACACCGGTCTGTTCTTGGTTAACGATCTCCTAGTTTTC |
Primers for UN77400 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TTCCCTTCTTCCTGCTCTGA |
60.065 |
20 |
TCGTTAACCAAGAACAGACCG |
60.154 |
21 |
247 |
465 |
711 |
| 2 |
CCCTCTTTCCCTTCTTCCTG |
60.175 |
20 |
TCGTTAACCAAGAACAGACCG |
60.154 |
21 |
253 |
459 |
711 |
| 3 |
CCTCTTTCCCTTCTTCCTGC |
60.319 |
20 |
TCGTTAACCAAGAACAGACCG |
60.154 |
21 |
252 |
460 |
711 |
|
|
