Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
1 |
TTTGACTATTTCTTTTCCTTTGATTT |
58.074 |
26 |
AATTGGATTTTCTTTCTCTAATTGTTT |
58.36 |
27 |
190 |
43 |
232 |
2 |
TTTGACTATTTCTTTTCCTTTGATTT |
58.074 |
26 |
AAATTGGATTTTCTTTCTCTAATTGTT |
58.36 |
27 |
191 |
43 |
233 |
3 |
TTTGACTATTTCTTTTCCTTTGATTT |
58.074 |
26 |
ATTGGATTTTCTTTCTCTAATTGTTT |
57.246 |
26 |
189 |
43 |
231 |