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Simple sequence repeat (SSR)
>UN00850
GGGCCGAACCAACTAACCCTCAGGGGACTCTTAGAAAAAAGAAAAAAAAAGCCTCAAAATCCTCTCTAGTCTTCTTCTAGAAACACCTTCTCTC
TCATTTAGAGTCTTTTCAAAAAAAAAAATGGCGGTTACATCTTCTAGAACCAGATCAACCGGTACTGCGCTACGCTCTTCTTCAGGCTTCGCTT
CCTCCACAACCTCAACCTTCTCGTCCCGTTCTTCAGCTTTCTTCAGCAACCAACAACAACAACAACAACAACAACCTCACCACAGATCCGCGTC
CCCGACGCGCGTGAACCTCTTCGCATCATCACCGTTAAAACAATCGTTCCGTTACTCGATCGATTCCAGATCTATATCCCCTAACCACCGATCC
ATCGCCGTTTCGAAGCCAAGCAGTGGTAGTAAGATCCCCGATTCGAGGAGGAGATGCATGTGCTCGCCGACGACTCACCCCGGTTCGTTCCGGT
GCGCTTTACACAAGAACGTGGCGAATCCCCACGGTCAAGGCGCCGTCTTAAGTTTATGTTTTGTTTTGTTGGGTGAAGATGAATTGTACGTAGT
AGTACTTCATTTGCTATTGAAGCGCACTGAATTTCCATAAGCTCGAAAAGTATCTAAGAACACTTAGTAGTAGAATTGGTTGTTAAAAATCAAA
CGGAACAAAGGGACATGGTTTTATAA |
Primers for UN00850 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CTTCTCGTCCCGTTCTTCAG |
59.982 |
20 |
GGAATCGATCGAGTAACGGA |
60.036 |
20 |
145 |
205 |
349 |
| 2 |
ACCAGATCAACCGGTACTGC |
59.997 |
20 |
TTTTAACGGTGATGATGCGA |
60.073 |
20 |
180 |
143 |
322 |
| 3 |
ACCAGATCAACCGGTACTGC |
59.997 |
20 |
TTTAACGGTGATGATGCGAA |
60.073 |
20 |
179 |
143 |
321 |
|
|
