 |
Simple sequence repeat (SSR)
>UN10020
GCCGCAGATTCTCAGATCGGATCCTTCGCGCTAGAGAGAGAGAGAGAGACAGAGATGCAGAGGGACGTGTCGAGCTGCAACACTTACAACTATG
GAGACGCTCACTACTGGGACGCTCGTTACGTGCAAGACGCGCTTTCCTTCGACTGGTACCAACGCTACTCTTCTCTCCGCCCTTTCGTCCGAAG
CTTCGTCCCTACTTCTTCACGAGTCCTCATGGTCGGCTGTGGCAATTCCCTTATGTCAGAGGATATGGTCAAGGACGGATACGAGGACATTATG
AACGTTGACATATCTTCAGTTGCTGTTGAGATGATGCAAACCAAATATGCTTCTGTTCCTCAGCTCAAATATATGCAAATGGATGTTAGAGATA
TGAGTTACTTTGCGGACGATTCCTTTGGCACTGTCGTTGACAAAGGAACCCTTGATTCATTGATGTGTGGGACTGATGCTCCTCTAAGTGCTTC
GAGAATGTTGGGTGAAGTTAGCAGGCTGATTAAACCTGGAGGGACCTATTTCCTGATAACGTATGGTGATCCGAAAGTGAGAATGCCTCATTTG
AATCCTTCTGTTTACCACTGGAAGATCTCCTTATACATCATACCAAGACCAGGATTTAAAAAGCCTGGTGACTGTAGCTCGTCGTCGAAGTCGT
GTCTTGAAGCTATACCATTGACAAGTGAAGGAATGTTGCCCCATGATTATGTTCTGGAGGACCCTGATTCCCATTTTATCTACATATGTAGAAA
GACGGATGCAGAAGTAGCTCAAATACCCTCATACCCATTAATGGAGGATGTTCTGTAGTGTGATGCTTGTGGATGGCGTAAAACAATGCACTGA
GTGTTACCTTCTTTGATCTATTGTTGTATTTTGTATTTTCTCTTTTGATCTATATCCCTTTTGTAACTCTTAAAGTGTACTCTCTGCGTCTCCT
TTATACGAATAAACATCTTACTTTTTTTT |
Primers for UN10020 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TTCTCAGATCGGATCCTTCG |
60.295 |
20 |
GAAAGGGCGGAGAGAAGAGT |
59.957 |
20 |
172 |
9 |
180 |
| 2 |
TTCTCAGATCGGATCCTTCG |
60.295 |
20 |
GTCCTCGTATCCGTCCTTGA |
60.073 |
20 |
268 |
9 |
276 |
| 3 |
TTCTCAGATCGGATCCTTCG |
60.295 |
20 |
TCCTCGTATCCGTCCTTGAC |
60.073 |
20 |
267 |
9 |
275 |
|
|
