 |
Simple sequence repeat (SSR)
>UN01234
TTTTTCTCCACTGAAATCAATCCCACAAGCGGAACTTCAAATCATTCCCACGATCTCGGATTCATCTCTCCGTACGAATACAAATACGAATGGA
AGCTTCGATTTCGTTTATTGGGTCAACTACAAAGCCGCCCAACCTTTCATCACTTACTATAAGCCCTTCTTCTTCTTCTTCTTCTTCCAACGTC
CTTCGTCGTCTCCCTGTTCATGTTCCTGTTCCTGCTTTGAGTTTGAAGAAAGTCTCAGTCTTTCCTCGAATCTCCCACCGTAACCACCGCTTAA
TCAGAGCTCAGTGCTCAGATGGATTGAGACCAGACGAAGACGATGGGTTTGTGCTAGAAGACGTCCCTCACTTGACAACCTTCCTCCCTGATTT
GCCGTCGTACCCAAATCCATTGAAGGAAAGCCAAGCGTACGCCATTGTTAAGCGAACCTTTGTCAGTTCCGATGATGTGGTTGCACAAAATATT
GTAGTTCAGAAGGGAAGTAAGCGAGGAGTACACTTTAGGCGAGCAGGGCCTCGCGAAAGAGTGTACTTCAGATCAGATGAAGTAAAAGCTTGCA
TTGTGACATGTGGGGGCTTGTGCCCTGGGATTAACACGGTCATACGGGAAATCGTATGTGGATTGAACAATATGTATGGTGTCAGTAATATTCT
TGGCATCCAGGGAGGATATAAAGGCTTTTACTCCAAAAACACACTGATTTTGACGCCTAAAGTGGTTAATGATATCCATAAACGTGGTGGCACC
TTTCTTCAGACCTCGAGAGGAGGGCATGATACAGCCAAGATTGTTGATAACATTCAAGATAGAGGAATAAACCAGGTGATGGGACGCAAAAGGG
TGCAGAGAAGATATATCAGGAAGTAGAGAGGCGTGGTCTTCAGGTGGCGGTTTCTGGCATCCCCAAGACAATTGATAATGACATTGCTGTGATT
GACAATCATTTGGGTTTGACACTGCTGTTGAGGAAGCTCAGAGATCTATTAATGCTGCGCACGTAGAAGTGGAGAGCGTG |
Primers for UN01234 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CCACGATCTCGGATTCATCT |
60.034 |
20 |
GAGCTCTGATTAAGCGGTGG |
59.978 |
20 |
244 |
48 |
291 |
| 2 |
CCACAAGCGGAACTTCAAAT |
60.11 |
20 |
GAGCTCTGATTAAGCGGTGG |
59.978 |
20 |
269 |
23 |
291 |
| 3 |
CCACGATCTCGGATTCATCT |
60.034 |
20 |
ATTAAGCGGTGGTTACGGTG |
59.883 |
20 |
236 |
48 |
283 |
|
|
