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Simple sequence repeat (SSR)
>UN12280
GGGAGGTACCTCTCTCATTCATCCTATCCCTTTCTCAAATCAAGTAAAACTTTCTCAGGTTATAATGAAGTCAGAGGTACTGAATCGTCGATGA
ATCGCCTCCGTCGTCACCTGTCTCTCTCGCGTCTCTGTCTTCACCTCTCCCTCTCTCGCGCCCCCCAAGACGCAGCAACAAGAAACAAATGCAG
TGGTCATAGTCGTCAACCTATCTCCACCTCCACCTTCTCTCAGTCAGAAACTCGTCACCCCCCTCTCTCTCTCTCTCTCTTTTGATTCTGATTT
CGTCTCTGTTTTTTTGGTAAAGGATCTGTCATGAAAGTTTGAATCTTTTTCGGTTTTGTGTATAGAAAACTAGGTTTCAAGAAGCAGACGCCGG
TCTGTAGATCCGTAAAACCTAGAGTTTAAGGAAAGGGACAATAGATGGAGTTTGAGTCGAGCAAGATAGAAGAGAGGAGATGGAGTTGGTATAG
AGGCAAGGTAAGAGAGACAGATAAGAAGTTCCGATGGTGGTGGAAGAGCACAGATTGACGGCGGCAAAGAGTTTGGTGCAGAGATAGGGTTAAT
TTTTTTTTAAGGTTAAACCGGTTTAGTTTTTTAGATTTCTAAACCAGGTTCACGTGTAAACCTTAGGTTAATTATGGTTTGGTTTAATGTTTGT
AATTAGTTTTTTGGTTTAATGTATTCTTTAATTATTTGTATACTGATTTTAGCC |
Primers for UN12280 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GACGCAGCAACAAGAAACAA |
60.035 |
20 |
TTACGGATCTACAGACCGGC |
60.096 |
20 |
229 |
163 |
391 |
| 2 |
CAAGACGCAGCAACAAGAAA |
60.172 |
20 |
TTACGGATCTACAGACCGGC |
60.096 |
20 |
232 |
160 |
391 |
| 3 |
CCACCTCCACCTTCTCTCAG |
59.828 |
20 |
TTACGGATCTACAGACCGGC |
60.096 |
20 |
180 |
212 |
391 |
|
|
