 |
Simple sequence repeat (SSR)
>UN01255
ATCATGAGGACTAGGACGGTGAAGCGAGAATCGGAAGCCACCGGAGAAGCCAGTGGCAGCAGAGACGCCGGCGAGTCGGAGAGGTTTAGGTCTG
TGAAAAGGGAGAAGAACAAAGGCGTAGCTACAACCTCCGTGCGAAACGACGAGCAGCCGACTCAGGGAGAAGAAGAAGAAGAACATGAACAGGG
AGTATCTGATCGGAGGGTTCTCAGATCTCAGTATCTCGCTCTAATCAACAAAATCAGCGATTCTAAAGATGATATGACGAGTGTTGATTCTGAC
AAATTCTCTAGAATTTTCAGTGAATTTGAGAACCTCCACCAGAAAGTTCAGAAGCCTAGGGAGCAAATTGCAGATGCAGAGGCGTTTCTGGATA
TAGCAAACACCATGTTGTCATCCGTCAAGTCTCAGTCTGCTAACGGCGTTTCTCCCGCTGAGTTTGTGAATGCTTTGGTGAATGGGTTTGGTCA
GGCTCCTCTAGGGATCGATGATGCTGATGAAACTGCGGCCTCTGTATCTATCAAATGGAATGATCTTGGGCTTGCTGTCTGTTCTACTGTTTTG
GTCTCGTGTGGCTGTTCCACTATGTTGGGACCTATGGACACTGAACTGAAGGAGAGGAAAAGGGCAGTCTACAGAAAACGTACAAAGCCTGGTG
AAGCGTTCGTCCCGATGAGTAGACGATACGCAATCAGAAGAGAAACTGATACAGACAGATATGGCGATATGTTAACATCTTACGCAAAGAGCGC
GTGGGGCTTGA |
Primers for UN01255 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
AGGGAGAAGAACAAAGGCGT |
60.247 |
20 |
TTTGCTCCCTAGGCTTCTGA |
60.088 |
20 |
250 |
100 |
349 |
| 2 |
AGGGAGAAGAACAAAGGCGT |
60.247 |
20 |
TCTGCATCTGCAATTTGCTC |
60.104 |
20 |
263 |
100 |
362 |
| 3 |
ACTAGGACGGTGAAGCGAGA |
60.012 |
20 |
TGAGATCTGAGAACCCTCCG |
60.34 |
20 |
209 |
10 |
218 |
|
|
