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Simple sequence repeat (SSR)
>UN12509
GGAGGTGTGTATATGAAAAAAAAAGAAGGAGATTGTTGATGCGTTTCCAACGACGACGTGATCGTCCTCTCTCCTCCTCCTCATCTATTATTAC
AATTTCATCTCCTCTTTTCCCAGATTATCATCATTGTCTTTGTCTCTCTCTAGATTCTGGCTTCTCCTCGTATCAGAACCAAGATCCTCTGCTC
TAATACCGACAATGGCAACGGGAAACAAAGGAAGACAACTGCCAAAATTTGGGGAATGGGACGCCGCGACTAATCCAGGCGCCGCCCAAGGATT
CACCGTCATCTTCAACAACGCTCGCGACGACAAGAAGACCAAGAAAACAGCTGTTGCGGGACCTGAGAGTCTTGTTACACCTCCAACAAACAAC
GAGCCTCAACAGAACAACAACCGTCGTCATCATCGTCATCAACAGAACAACAACCATCGCAAGAAACGTAATTCGTCGCCACCCCCACGTGAAA
AGAAGAAATGGCTTTGCTTCTATTGAATCTGGTTCGGGCTCTGTTTTCAAAAAACGCAGTGGTAAGAAAGGAAGCAGATCGGTTCAGCAGAGTA
GTTTTCAACAAATGACAACGACAAAAACCTTCTGTTCGTGTTAGATTGTTGTTAACTTCAATTCTTCTTCTTCTTCCTCTTCTCGTCGAGTTTC
TCTTTTCAACTTTGTTTTGGCCACACATTTGGGTGTATTAATTAAATATACATTATTGTTCTTGCTGCTTCTTGGTTTGATTTTATAAAATGTT
GACAATGTTCTTTAC |
Primers for UN12509 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CCCCACGTGAAAAGAAGAAA |
60.081 |
20 |
TGTGGCCAAAACAAAGTTGA |
60.127 |
20 |
225 |
459 |
683 |
| 2 |
ACAGAACAACAACCATCGCA |
60.16 |
20 |
TGTGGCCAAAACAAAGTTGA |
60.127 |
20 |
266 |
418 |
683 |
| 3 |
CAACCATCGCAAGAAACGTA |
59.729 |
20 |
TGTGGCCAAAACAAAGTTGA |
60.127 |
20 |
257 |
427 |
683 |
|
|
