 |
Simple sequence repeat (SSR)
>UN13539
GGACACGCATCAAAAAAACTATGAAGTCATCACGCATTGATTTTTCCATGTTCAACAGTCAAGGACAACACGATGAGGACAACATGTCACGTCT
TCCATGGAAGAGGGTCAGAGACGAGGACGAAGAGCTTGCCAACTGTCTTGTGTTGCTGTCCAATTCAGGGAGTGATTACAACAAGGGGCATGTA
CACATCAAAGATAAAACCGTGAAAAAACAAAAAACCGCACATGTTTTCCAATGCAAAGGTTGCAAGAAAGTGTTTGCGTCGCATCAGGCATTGG
GTGGACACAGGGCAAGTCACAAGAAAGTGAAAGGTTGTTTTGCCTCTCAAGATAAAAAAGCGGAGGAGGAAGAAGAGGAGGAATACAAAGTGGA
CGACGACGATGAGGAGGAGGAGGAGGAGGAAGAGGACAAGCCTCACATCATCGCCAGAAAGAAAACTAACGCTCACGAATGCACGATCTGCCAT
CGCGTATTCTCATCAGGACAAGCCTTGGGAGGTCACAAGAGATGTCATTGGTTAACACCTTCCACTAATTATCTTCGTATGACGCCACTAAATG
ATTCTTCTACTCATCACCATCATTCTCAGCCGCTAGATCAGCCATCTCTAGATCTCAACTTAGCTTGCGTTGATCCAACGGTCATGAGCGTAGG
GAGAGATGGTGGTGGCAACAATCTCAATGCTACTTCATCTAATTCATGGTTAAAACTCGCTAGTGGTGACTGGTCGTGATGACTGACTAATAAA
TTGAGTTGATGATTTTATATTCATTCGCTTATATACACATAAATATACTCACATACAATTAAAGTACATTAATCAATTTATAAATTTGTAATGG
ATTTTATTATATTAATATACGCTTCACGTTTCAGAGAGCCTAGTTTATTTAGGGGATGTTAGAACTTTGACATTGTTGG |
Primers for UN13539 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TGCAAGAAAGTGTTTGCGTC |
60.035 |
20 |
TTCTTTCTGGCGATGATGTG |
59.799 |
20 |
191 |
249 |
439 |
| 2 |
AAAAGCGGAGGAGGAAGAAG |
59.955 |
20 |
TTCTTTCTGGCGATGATGTG |
59.799 |
20 |
102 |
338 |
439 |
| 3 |
AAGCGGAGGAGGAAGAAGAG |
60.088 |
20 |
TTCTTTCTGGCGATGATGTG |
59.799 |
20 |
100 |
340 |
439 |
|
|
