 |
Simple sequence repeat (SSR)
>UN00147
GGGCAACACCCTTGATTTTCCTATTTTACGAGTTGGACTTGGAAAGAGGAGAGAAGGAATCGAAACCGGGGCTCCGTTAGCAAAAAGGTATAAT
AGCTGAATGTCTATCGGTTCTCTTCCCCAGAGAGAGAGAGACTGTAAACCCTAATCATCACCAATTCGAATAGATGGAACAGTACCCTTATCAA
AATCCGTTCGAAAGACGCCCAATCGTGAAATCCAAGGCTCCTGCTGTTAAGTGGATCAAGGAGTGGGTTCCACAAGATATTGTTGCTACAGGTG
GGAAGTGCTATCTTCACAAATGGGTCACAGAGGAAACGGTGAAGAGACTGAAAGAAACAGAGAAAGAGCCTACTACTGATGTTCCGGACCCTGA
GCCCGAACCTACTTCTGAGATTCTGTTTCTATGCAGTTACGAGGGTTGTGGAAAGATGTTCTTTGATGTTAGTGCCTTGCGCAAGCATTCCCAC
ATCCATGGTGAAAGGCAGTATGTTTGTGATTACCCTGGTTGTGACAAGAAATTTCTTGATAGTTCAAAGTTGAAGAGACACTGGCTCATTCATA
CTGGGGCTAGGGACTTTGTTTGCACTTATGAAGGCTGTGGAAAGGCATTCTCCTTGGATTTTAACCTCAGATCTCACATGAAGACTCATTCACA
AGAAAACTATCACATCTGTCCCTACTCTGGTTGTGGAAAGAGATATGCTCATGAATACAAGCTTAAGAACCATGTTGCTGCCTACCATGAAAAG
AATGCTGCTGGAGACACGCCTAAATACACCCCACCCGCAGAGAA |
Primers for UN00147 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GGCTCCGTTAGCAAAAAGGT |
60.612 |
20 |
CTTAACAGCAGGAGCCTTGG |
60.008 |
20 |
170 |
70 |
239 |
| 2 |
GGCTCCGTTAGCAAAAAGGT |
60.612 |
20 |
CGTTTCCTCTGTGACCCATT |
59.966 |
20 |
251 |
70 |
320 |
| 3 |
GGCTCCGTTAGCAAAAAGGT |
60.612 |
20 |
AGCCTTGGATTTCACGATTG |
60.074 |
20 |
158 |
70 |
227 |
|
|
