 |
Simple sequence repeat (SSR)
>UN16012
TTTTTGAGAACCAAAAAAAAAACGAAGGAGGAAAAGGGGATTTGGTTTTATTGGAGGGTGTGAATTGTTGTTTAGCAGCGCCGAGATTACAAAT
TGGAGGGTGAATTTGAGCAGAGAATGGAAGAAATAAGATCAGCGATGGAACAGCAGATGGATCTGATGGCGGATCTGGTCCACAAACTCTCCGG
AGAGCTCCGTACCGGATTGCAACCTGCCTACGAGAATTTCATTGGTTTCTTCCACGCCATTGATTGGAAGGAACCTTGGATTATGGGATTAATG
GCGTTTCATGCTCTGGTGCTAATTGTGACTCTTGTTTCGAGAAGGCATCTCAACTTCCACATGTTCTTGTTCTTATTGTCTTTGGGTGGAGTTT
ACTTTGCAGAGAGTCTCAACCGAGTCTTGAGAAAGAACTGGAAGAGCTTCTCAACTCAGAATTACTTTGACCCCCACGGAGCCTTCCTATCTGT
TCTCTGGTCAGGACCACTTTTGGTCATTGCAATCATAATCCTGATAAACACACTGTTCTCGCTTTGCTACCTTATTGTAAAATGGAAAAGAGCT
GAGCTCAGGCATCGTGCAAGGCTTGCTCGTCCCAAGCAGGAATAATAGATCCCAACTCTCTCTCTCTCTCTCTTTTCTTTTTTCTCGTATCACA
TCCTTTTTGTCACATATATGTATACTCTCCTCCCTCATTTTGCAATGCAAACTGTTAGTTTTCTTTTCTTTTTTCTTTTTCTTTTTTTTTTGTT
AACAGCAAACAGTTAGTTTTCTATTTTATGTATTTCGTTTTG |
Primers for UN16012 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CTCGTCCCAAGCAGGAATAA |
60.206 |
20 |
TGCAAAATGAGGGAGGAGAG |
60.331 |
20 |
113 |
590 |
702 |
| 2 |
GGACCACTTTTGGTCATTGC |
60.362 |
20 |
TGCAAAATGAGGGAGGAGAG |
60.331 |
20 |
222 |
481 |
702 |
| 3 |
TCGTCCCAAGCAGGAATAAT |
59.528 |
20 |
TGCAAAATGAGGGAGGAGAG |
60.331 |
20 |
112 |
591 |
702 |
|
|
