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Simple sequence repeat (SSR)
>UN19991
GGAAGACAAAATGTTTTCAAGACAAGAGATAACACAAATGATTGCAGCCACATGCAGGATGATAAACTCCCCACCAGAGTAAGCAAATTAAAAG
GCTTATCTTCTTCTTCTTCTTCTTTGATATCCCGTAGCTATAGCTTGAAACTCAAACAAACCAATGGCTATCTCAAAAGCTTTTCCATTGCTTC
TCGTCTTGTTGCTAGTCCTCAACTTAACATTCTCGTTTTGTCATGCCGTTAAGCAATGCCCTCCTCCCACAAAACAATCATCCATGAAATGTCC
AAGAGACACGGTCAAGTTTGGAGTGTGTGGGAGCTGGTTAGGTCTAGTTCATGAGGTCATCGGTACACCACCGAGCCAGGAGTGTTGCTCACTT
GTCAAAGGTTTGGCTGATCTTGAAGCTGCACTTTGTCTTTGCACTGCCCTTAAAACCAGCCTACTCGGAGTCGCTCCGGTTAAACTTCCTGTTG
CTCTCACTTTGCTTCTCAACTCTTGTGGCAAGACTCTTCCTCAGGGATTCGTCTGTTGATCTTGAACCAATTGTTATCTTGTAAAAGAATTGTT
TCTTTTTTTTCTTTTATTGTATTTCATATTCCTGTTTTTCTTACTGAACATTTATGCAACATTTATTTTCTATATCAAGATCTTTTATAT |
Primers for UN19991 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GCAGGATGATAAACTCCCCA |
59.894 |
20 |
TTAACGGCATGACAAAACGA |
60.111 |
20 |
187 |
54 |
240 |
| 2 |
TGCAGGATGATAAACTCCCC |
59.894 |
20 |
TTAACGGCATGACAAAACGA |
60.111 |
20 |
188 |
53 |
240 |
| 3 |
GCAGGATGATAAACTCCCCA |
59.894 |
20 |
AGCTCCCACACACTCCAAAC |
60.159 |
20 |
264 |
54 |
317 |
|
|
