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Simple sequence repeat (SSR)
>UN20000
CTGGAAAATTAGCATGTAGATTAAAGTTGTTATCAATACTCAAGCTGCTTGTGTTCAAATAAAGCATACAAACTATAAATGAATGCCAATAAGC
TATGGTTTTGGTATCATGGCTTTGTAAGCTCATTCCAACACCCAATGGAGTAATCTTTTAAGTATAGATTGACAGCTTCGAGAAAGAAACATAC
ACCAAATCATATAAGTTTTTTATGAGAATTGAGTAACTCTCCAATCTTAGACGAAGATATCGTCTAACAGACAGCAGCAACAGAGAGTAGCGAG
ACAGCCACGGAGGAAAGAGGTGCAACCAGAGTTAGAGTCCTGGTAATAGTGATGATCATGATGACACTGATTGTATTGCTGCGGCGGAGGTGGA
GGAGGAGGAGGAAAGCCACCGGAGAAGTACTCCTGATATCCCCCTTCGTAAGGAGGACGTGAAGGCGGTCGTGGTGGTGGATACGATGGATATC
CGTGTGGGTGTTGAGGATATCCTTCGTGATGGTGCGACGGAGGATATACCGGAGGCGGGGGAGGATACTGAGATTGGTAACCTGGAGGAGGATA
CGTTTCAGGCGGAACCTTCCCGTAGTCGTAGCTCATTATGATATCTTCTTTGAGAAAACTTGTTGCTTGGTCTGAGGGAGAATCC |
Primers for UN20000 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GGAAAGAGGTGCAACCAGAG |
59.844 |
20 |
CCTTCACGTCCTCCTTACGA |
60.246 |
20 |
148 |
294 |
441 |
| 2 |
AGGAAAGAGGTGCAACCAGA |
59.844 |
20 |
CCTTCACGTCCTCCTTACGA |
60.246 |
20 |
149 |
293 |
441 |
| 3 |
GAGGAAAGAGGTGCAACCAG |
59.844 |
20 |
CCTTCACGTCCTCCTTACGA |
60.246 |
20 |
150 |
292 |
441 |
|
|
