 |
Simple sequence repeat (SSR)
>UN20116
GGATTTTCGAAAAAAGAAGAAATTCATTCTATTCGCGTGGTTTTGAGGAGACGAGGAAGGGAACAAACGCATAACGAAGACGACGATGGGTGAA
GCCGTGAAGGATGGAAGAGAAGAAGTGATTCAGGCATGGTACATGGATGATAGCCAAGAGGATCAGCGACTTCCTCACCACAAGGATCCTAAAG
AGTTCATCTCTCTCGACAAACTTGCAGAGCTTGGAGTCCTTAGCTGGAGACTTGATGCTGATCACTACGAAACCGATGAGGAGTTGAAAAAGAT
CCGCGAGTCTCGTGGCTACTCCTACATGGACTTCTGTGAGGTATGCCCTGAGAAGCTTCAAAATTATGAAGAGAAAGTAAAGAGTTTTTTCGAG
GAACATCTTCACACTGATGAAGAGATCCGTTACTGCGTTGCAGGAAGTGGCTACTTTGATGTGAGGGACCGCAACGAAGCTTGGATCAGAGTTT
GGGTGAAGAAGGGAGGAATGATAGTCTTGCCTGCTGGAATTTATCATCGCTTCACCGTGGATTCTGACAACTATATCAAGGCAATGCGTCTTTT
CGTGGGTGAACCGGTGTGGACACCATACAATCGTCCACACGACCATCTTCCTGCAAGGAAAGAATATGTTGATAATTTCGTGAACAAAGCCGTT
AACGCCTCTGCTTAGAGAGCTCTCTCTCTCTCCCTTCTAAAGAAACATGATCTATAATAAACTTGGCCTGCAATGAAGAGAGTATGCATGTGGA
GAATGATTGCTTTGGTATGCATGTCTTGTAAACTTAATCACACTCTGCTCGTCTTTATTGACCATCTTTGCCGTATCCGTTTTACGAAAAATTG
TATCGAGTCAGTTCGATCATATATTTTCATGCGTACGTTTCTTTGTGAGCAACTAAGGAAACATAATAAGAGTTGTTTATTGCTGCGTGGATAA
TTTAATGAGCCTTATTATATATGTCG |
Primers for UN20116 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GTCCACACGACCATCTTCCT |
59.969 |
20 |
TTTCGTAAAACGGATACGGC |
59.964 |
20 |
245 |
597 |
841 |
| 2 |
CCGTTAACGCCTCTGCTTAG |
60.032 |
20 |
TTTCGTAAAACGGATACGGC |
59.964 |
20 |
188 |
654 |
841 |
| 3 |
GTCCACACGACCATCTTCCT |
59.969 |
20 |
AAACGGATACGGCAAAGATG |
59.96 |
20 |
238 |
597 |
834 |
|
|
