 |
Simple sequence repeat (SSR)
>UN20130
GAAAAAAAACTGGGTTGAGGGAGGAGAATCGTAGTCATTACAGAAGTAGAAGAAACCCAATAGTCTTGAGTAGAGATACAATGGAATATAAGAA
AATCTGAAAGGGTGTTTTTGCTGCTATACAGAACCAAAATGGGGGGTAAAAGATTTCATTGTCTTCGTGCAAATATGAGAAGCCTTCATCATCA
ATCAATCATCTTCCGGATGAGGAGCTTGATCATCATCATCATCATCATCATCCGTTGACTTCTTTTTCTCGTTCCTCTCATTCCATCTCGCCCA
AGCCCACTTCAGGACAAGAAACCCTCCAAGTGCATATACCTGCCAAATGACGAGGGAATTGGAAGTGGTCCCGGAGCTAGGCTCGGCTTCAACC
TTGAGTGGCTCAGTGCTCTTGGGAGGATTGTAAGGGAAAGTGACCGTCGAGTTCTGAGTCTCCTTATCTCCTCCCGCGGGTTTAGGACCACCAT
CGGCAACGTTAGCAGAAGAAGCAGGAGACTTAGGTTTGAGGAAGGGGAACAGGTTGACGACGAAAGAAGGTAAGTTCAGCTGAGGAGGAGGGGG
AGAATCGGGTTCAGCCATAGTTGAGGTGAGGATGGCACCGCTTAGGGTTCTGAATCGACTGGGGATTTTTTCTTAAGGATCC |
Primers for UN20130 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
AAAAACTGGGTTGAGGGAGG |
60.332 |
20 |
GCGAGATGGAATGAGAGGAA |
60.303 |
20 |
275 |
5 |
279 |
| 2 |
GAAAGGGTGTTTTTGCTGCT |
59.359 |
20 |
CCTCGTCATTTGGCAGGTAT |
59.955 |
20 |
238 |
100 |
337 |
| 3 |
GAAAGGGTGTTTTTGCTGCT |
59.359 |
20 |
TTGGAGGGTTTCTTGTCCTG |
60.081 |
20 |
212 |
100 |
311 |
|
|
