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Simple sequence repeat (SSR)

>UN20140
GGAGAGGAATCCATTTCCTGTTTAGATTCCGAGTGACGCCTCTCTCTCTCTCTCTCTCCCCTCTTCTCGATTCGTTTCTTTGCCGGAGCTCGCA
GTTTGGTGGAGAAAGATGGCTCCCAACGATCCTAAAAACGGTGGCGGCGGTTTCTTCGCTTCTCTTGCTTCCTCAATCTCCAATTTGGGATCGG
CCATGACCAAATCAGTTAACGGTTTGGTTGGCTATGAGGGACTTGAAGTTATTAACCCTGAAGGAAGTACAGAAGATGCAGCCGAGGAAGCAAA
CAGAGGAAGATGGAAGCAAGAGGATCGAGATGGTTATTGGAAGATGATGCAGAAGTACATAGGATCTGATGTCACATCAATGGTTACCCTTCCC
GTGATTATCTTTGAACCTATGACAATGCTCCAGAAAATGGCTGAGTTGATGGAATACTCGCATCTATTAGACATGGCAGACCAAACTGAGGACC
CTTATATGCGCATGGTGTATGCGTCATCATGGGCTATCTCTGTGTACTATGCTTTCCAACGTACTTGGAAACCGTTTAATCCAATCCTCGGAGA
GACTTATGAGATGGCTAATTACAATGGTGTTAACTTCATATCTGAACAGGTCAGTCATCACCCGCCAATGAGCGCTGGCCATGCTGAAAATGAG
CATTTCACTTATGATTGTACATCAAAACTGAAAACAAAGTTTCTGGGCAATTCCATTGACGTTTACCCAGTAGGAAGGACACGAGTGACACTTA
AAAGAGATGGAGTGGTTCTTGACTTGGTACCTCCTCTGACCAAAGTTCACAACCTAATCTTTGGACGAACTTGGGTGGATTCTCCTGGAGAAAT
GATCATGACCAACCTGACCACTGGTGACAAAGTCGTGCTTTACTTCCAACCATGCGGCTGGTTCGGATCTGGTCGATATGAAGTGGACGGATAT
GTCTATAACGCCTCTGAGGAGCCCAAGATACTCATGACTGGTAAATGGAACGAGTCGATGAGCTATCAGCAGTGTGACGGTGAAGGTGAACCTC
TCCCAGGCACCGAACTAAAGGAGGTCTGGAAACTCGCTGATGTTCCAAAAGATGACAAATACCAATACACACACTTTGCTCACAAGATCAACAG
CTTCGACACTGCCCCGAAAAAGCTGTTGCCGTCTGATTCACGGTTACGCCCTGACAGATACGCACTAGAGATGGGTGACGTGTCCAAATCCGGA
TATGAGAAGAGCAGCATGGAAGAGAGACAGAGAGCTGAAAAGAGAACCCGCGAAGAGAAAGGCCAAAGCTTTACTCCCAAGTGGTTTGATATAA
CCGAAGAAGTCACTCCTACACCATGGGGGGATCTCGAAGTTTACCAGTTCAACGGAAAGTACTCAGAACACCGGGAAGCTGCGGATAGCTCTGA
AGATAATACGGACGACCCTAAGTCAATCACATTCAACCCATGGCAGCTTCAAGATTTGTCTACCTAATGAATCGCTCCAAAAGATAAGAAAAAA
AAAAGATCAAAATCTTTTGGTAACAGATGTTGTAATTCTTATTCTCAGCTTTCTTTACTTTCTCTAGTAGTAGTTTTACAAACTTTTATTGTTC
TTTTATATTTAAATCAAATCTCTCTTTCATTCTCGTTGCCATAGCCATCTTCTTCTTCTTCTTCTTGGGGCATGATCAAACCCTCACCAAACCA
TCTTGGAGGATCGTATCCAGACCCACCAAAAGGATTGCAGCGGCAGAGACGCCATGTGGTGAGAACAGTGCCTTTGAGAACTCCATATCTTTTG
TAAGCTTCCATAGAGTACTCACTGCAAGTTGGAACGTAACGGCGGCTTCTAGGTAACACCGGCGATATCTCCCTCTTGTAGAATCTCAGCATTG
CTAAAGCTACTTTGACTCCCACACTCTCCTGCTCTTCCCCATCTTTAGGAGATTCAGGGTCTGGACTCGAAGGCGTTGATTTCGCGGATAAGCA
ACGGAGCTGATGCTTGTTCGTATGAGAAAGATGATGAAAGCTCAAGCGAGACGTACTGAATTTCAAGTGAATTTTAGGAGGATTAGGGTTTTCA
GAGATTAAGTGGGTTGCATAAGGACGATGAACGGAGACGACGACGACCGCCATCAAATGCAAAACTCATACAAACAAAGCTTTTACTTTGAACC

Primers for UN20140 SSR

Primer pairForward primer (5'-3') Reverse primer (5'-3')Product
SequenceTemperatureSizeSequenceTemperature SizeSizeStartEnd
1 TCCATTTCCTGTTTAGATTCCG 60.305 22 CTCATAGCCAACCAAACCGT 59.993 20 217 10 226
2 CCATTTCCTGTTTAGATTCCGA 60.305 22 CTCATAGCCAACCAAACCGT 59.993 20 216 11 226
3 TCCATTTCCTGTTTAGATTCCG 60.305 22 GTCCCTCATAGCCAACCAAA 59.933 20 221 10 230