 |
Simple sequence repeat (SSR)
>UN20219
CAATTCCGCGTTCCTCTCTCTGTGTACAAGTCGAAAAAGATCTAGAGAACAGAATCCTGGCGTAAATCTCGTTAGGGTTTTTCAGCTTTTTAAA
TAAAAGCGCAAAAAACATTTTGATCTCTCTCTCTCTCAATGGCGGGTTACAAAGCAGACGATGAATACGATTACCTTTTCAAGGTTGTTCTCAT
CGGTGACTCTGGTGTTGGAAAGTCCAATCTGCTTTCACGATTCACAAAAAACGAGTTCAGCCTCGAGTCCAAATCAACCATCGGAGTCGAGTTC
GCGACTAGGAGCTTGAATGTTGATGATAAAGTCATCAAAGCCCAGATTTGGGATACTGCTGGTCAAGAAAGGTACCGAGCCATCACTAGCGCAT
ACTACCGAGGAGCCGTGGGAGCGCTTCTCGTCTACGATGTAACCCGACACTCCACGTTTGAAAACGTGGAGACGTGGCTCAAAGAGCTCAGAAA
CCACACCGATCCAAACATCGTAGTCATGCTCGTGGGGAACAAATCTGATCTCCGCCACCTCGTGGCTGTTCAGACAGACGATGCCAAGTCATTC
GCTGAGAAAGAGTCTCTTTACTTCATGGAAACCTCGGCTCTGGACTCTACCAATGTTGAGAACGCTTTCGCTGAAGTCCTCACTCAGATTCACT
GCGTTGTGAGCAGAAAGGCCATGGAAGCTGCGAGCGAATCCGCTAATGTTCCGTCCAAAGGAGACAAGATCGATCTTGGTAAAGATGTTTCTGC
TGTGAAGAAAGGTGGATGCTGCTCAAACTAATAATAACAGAGGGGAACCACTTGGTTACGTGTTCAAAAGTGGAATATGAGAGATTGCTTTAGG
TTGATTGGTTAAATGCTCTTGTGATGTATTTCTGTTGGTTTTATAAATTAATAGATGTTCTTGCCTGAGAAAATGATTTTGAATTTTGATGTTT
TTTCAAATACCCGGAACTATGCTATCTAAGAAAATATATATTCTCTCTGTTTCG |
Primers for UN20219 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
TGGCGTAAATCTCGTTAGGG |
60.089 |
20 |
TCGAGGCTGAACTCGTTTTT |
59.993 |
20 |
197 |
58 |
254 |
| 2 |
TGGCGTAAATCTCGTTAGGG |
60.089 |
20 |
TTTGGACTCGAGGCTGAACT |
59.989 |
20 |
204 |
58 |
261 |
| 3 |
TGGCGTAAATCTCGTTAGGG |
60.089 |
20 |
CTCGACTCCGATGGTTGATT |
60.073 |
20 |
222 |
58 |
279 |
|
|
