 |
Simple sequence repeat (SSR)
>UN21333
GGGTTCACGTTTCTTCCTCCTCACACCGGCATAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAATTGAAGAAAGCAAGCAGGAAACATAGAGAT
TCTTTCTTGTCCTTTCAGTGAGGTTTTTGGGTTTAGCGAATCGATTGATTTAGAAGGATGACGAGTTCTGCCGAGTGTTGGGAATCCTCCGGTG
AGAGGAAGAAGGTGCTTGAGAAGAAGCCGAGCTTCTCGCAGACATGTAGTCGATTGAGTCGTTATCTCAAGGAGAAGGGTAGCTTCGGAGATCT
CAGCTTAGGGTTGACATGCAACCCCGACCTCCATGGAGCTTTTGGTGTGTCACGTGAGCCCACGATGATGAATCTCTTCCCTTCTTCGGCTGCT
CAAGATGTTAAACCACCGAAGAGTGATGTGATTCCTCGCCAATCAAGCTTTTCTTCTTCCTCTTCCCCTGGAGGAGCCAAGGAAGAAGTCGATA
AGATCATACACATCAAATCTGTGAAGGTGGAGTCTCAATCTGCGCCATTGACCATATTCTACGGCGGTCAAGTTATGGTGTTTGATGATTTTCC
TGCTGAGAAAGCCAAGCAAGTCATTGACTTGGCTCACAAAGAAAGTGCTAAAAGCTTCACATCTGAACTGAACAGTAGCCAGATTGCTTATACT
CAAAACATTGCCAATAATCATAAAGAGATTGCTTCCACTACCCCAAATCCAGTTTCTATTCATGTGAAACGGCTGCACCAGAGCCAATCCAAGC
CAACAAATCCTCTTTAGCTTGCGGTACGACTCCCAATTGCTAGAAGAGCTCACTTCATCGATCCTTGAGAGA |
Primers for UN21333 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CGTTTCTTCCTCCTCACACC |
59.697 |
20 |
TCAATCGATTCGCTAAACCC |
60.038 |
20 |
135 |
8 |
142 |
| 2 |
GTTTCTTCCTCCTCACACCG |
59.697 |
20 |
TCAATCGATTCGCTAAACCC |
60.038 |
20 |
134 |
9 |
142 |
| 3 |
CGTTTCTTCCTCCTCACACC |
59.697 |
20 |
CGATTCGCTAAACCCAAAAA |
60.068 |
20 |
130 |
8 |
137 |
|
|
