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Simple sequence repeat (SSR)
>UN21411
GGACCCTAGTTTCCACTGCTGAACTGCGCCGCGACATATATATCTTTAATCCTCTCTAGCAGGGCTGTTGTAGTGACAGTTTCTATCGGAGAAG
ATGAAGTTCAACGTCGCGAATCCGACTACTGGTTGCCAGAAGAAGCTTGAGATCGATGACGACCAGAAGCTCCGTGCTTTTTACGACAAGAGGC
TCTCACAGGAAGTTAGCGGAGATGCTTTGGGCGAGGTGAATTTTCTACCTACTACTCGTTTGGAGAAGCTAAGGCGATTGGAGAAGTTACAGAG
TGTTGTATCAGCCATCTCTTCTCACGGCTTACTTTCTTCTTCGTCAGCTTCAGATAATGAATCTTCTTCTTCTTCTCGATTCCTTTCGGAGCTC
GTACTTTTCCTGCTACAACCATCCGGCGACCTTGATATTGAATCGAAACTCGTTTTGATCTCTGATTTCATACCTAAAGTATCTGGAACTTTCC
TCGATGAGATATCTAGGTCAGTTCAGCGAGAGGATGAACCTAAACCACTAATCACCAGTTCAGCAGAGGTCAAAAGAGTTGTGTGAAGAGGAGT
TTCATGGACAGGAGGGAGTGAACTCCTCATGGTGGTGGTGATGATGATGCCAGTTTTTTTGTGTGTGTTGTTTAACAATTGAGCGAGCGAAAGA
GACAAGAAGGTTGAGAAGATTCGGAAGAGTAAGAGAAAACTTGTTTTTTATTGTATTTTTTTTGTTTCTTATGTAACCGACTTTTTTTTTCTCT
TTTTGTTTTTTCTTTAATATTTTGGATATAATATTTAACTCTTTTAAATTTAAATTTATCTCATAAGAAATAATCGCAGC |
Primers for UN21411 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
AAGCTCCGTGCTTTTTACGA |
60.018 |
20 |
ATTCAATATCAAGGTCGCCG |
59.923 |
20 |
259 |
161 |
419 |
| 2 |
GAAGCTCCGTGCTTTTTACG |
60.018 |
20 |
ATTCAATATCAAGGTCGCCG |
59.923 |
20 |
260 |
160 |
419 |
| 3 |
CAGGAAGTTAGCGGAGATGC |
59.978 |
20 |
ATTCAATATCAAGGTCGCCG |
59.923 |
20 |
226 |
194 |
419 |
|
|
