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Simple sequence repeat (SSR)
>UN21716
GGGAGAGTGAGAAATACAAGGAACGTTGCAGAGCAGTTTCCGATTCTCATCTCTCTCTCTCTCTTCCACCGATTAGGGTTTGCGTTCTCTTCTA
CATTACTCCGTGTGTGCGTGAGTGCCTCGGTGTCCTCCTTGAAATTGTTCCAGAGAAGAGAGATAGTCATGTTTACTGATTCGATCACAGACTC
TCAACCATTCAATGAGTCTCCGATTTCGAGAGATTTCGTCAAGAAACGGAAGGCGAATTGGTTTTGGAAACTGAAGCAGTGGAAGATTGATGCT
CGTCGCAGGCAATGGATTTACCAATGGAAGAGAGCAAACGTAGGTGAAGTAGGGCTGAGATCTTTGCGTGACAAGCTGATTGATACAAAATCAG
TCAGAGCTTGGAGTAATGATGATGATGATCATGATGGACTACGATGCTACGGATTCGCCAAATGTGATGATGATGATGATGTTGTCGATAGTGA
TGATGAGACTGATCTCTCTGCTAGTAGCCCCACGAGTGTCCTCAAGAACAAAGATTCGGATAGTAATAAGAGTGTGCACGACTGCTTCTGCTGT
TCTAAGCAGATCACCGAGGAAGAGGAAGAAGCTTTTGATGATGCGTATGACAATTGGGAAGATTTTAGCGATGCCTTGAACAGCTTTGAGAGCG
AAGGGACTTTACAGCATGAAGATAGCCAGAGGAAGAAGTCTTCTTCTCATAAAGACAAGTGCTGCAAGCAAGAAGCTTCACCAGAAAAAGCGGT
TCATCATCATCACAGAGTCGACAGTAGAAAGAACAATAAAGACAAGTGCAAGCAAGAAGATTTACCAGAGAAGGCGATGATTCATCGTAATGTC
GGTAGAAAAAATAAGAAGAAGAAGTCTGATCAGAAGAAAGAAGGTGGTGGTGGTGACGAGATAAGTGAATGTCCTATTTGCTCTGAAGAGATGG
ATGCAACGGATCTAAGCTTCTTACCGTGTCCTTGCGGCTTTCGGCTATGTCTCTTCTGCCATAAGCAGATCAACGAAAACGATGGACGTTGCCC
AGCTTGCAGGAACAAGTATGAGCAGACGAGTAGTAACAGCGGTGAAGTTAGTTTCCAGCAACGTGGCCGTGGCACAGTTCGTTTGTCTCCTTCG
TTTAAAGGACTTGACATAGCTTAAGAGAGAAAGAAGATCGAGAGTTTTCATGGAAAGTTGATAAGCCTTTTACTTTAGTATAATAGTTTCTCAT
CAGGAACAACAGTTTTGTTTAGATTGTCATCTCAGCAATAAAGGAGTGTGGATTGGTTTAGGTATGCAACTATGGAAAGCTGCATTCTTTTTGT
AAGATTCGGTCTCATCACATTTTTTTTTACATACCACAACAGTTTTTTTTTACTACGGTAACTCATTTGATACATTGTAAAACTCTTGACATGA
TGATCATGGTGGTTACATTGTTATCATTCATCTCAGAACCCATCAGTTGCATTCTTCTTGTAGACTTGTGGACTCTATAGTCGCTCGTAAACAA
GTTTCTCGGACTTGATGAGCATATCTGTTGGTAGCCAAGCCGGTGCGGTTGGGATACCAACCCATCCAACAAGGGCATACTCTCCTGTCTCAAA
GACGCGAATGTCCAGAACCTCGCCGCGATCTTCATAGATGTAATCTAGTCCTTTGTAGAAAGGTGGATGACCAACTGATAAGTAATTGATGCTG
TTGAGATACTTCTCCTTGTCTACACGAACGATTTGACCCTTCTTCATCGAGTAAACTGGTTTCTTGGGAAGAGTTTTAGGGGTTTTGGTTGGAG
CTGGTGAAGCTTCCCCTTCGGATTTAGTCTGTGTGCTCTGTTTCTCATTCTCTGTCTCTGCTTTGACCCTGAACTGAGTCTGAGATCGAAGAGG
GAAGCTTCTAGAAGAAAGTGGCAGTGAAGAGGAGATTGTCGAAAGAGAAGAAAGCTGAGACAGAGTCGCTGAGAAAGCCATTGGAGAGACCACC
ACGCAAAATTTATCTGTGTTTTGTTGGGAGTTTGGGATAAGACCGCAATCC |
Primers for UN21716 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
AAGGAACGTTGCAGAGCAGT |
60.058 |
20 |
ACTCACGCACACACGGAGTA |
60.381 |
20 |
100 |
18 |
117 |
| 2 |
AAGGAACGTTGCAGAGCAGT |
60.058 |
20 |
AATTTCAAGGAGGACACCGA |
59.526 |
20 |
123 |
18 |
140 |
| 3 |
AGGAACGTTGCAGAGCAGTT |
60.058 |
20 |
AATTTCAAGGAGGACACCGA |
59.526 |
20 |
122 |
19 |
140 |
|
|
