Simple sequence repeat (SSR)
>UN22220
GGAACTTAAAATTATACATTTGTTTTTATTACTGGGAAAACTAAATACACAAACATCCAAACGTACAAACCTAAGGGGAGAATCAATTCAAGTA
TTTAAAACACTAAACACAACAACAACAACAACAACGACAAACAACAACTATGATGTAGATGAGAATCAATATGAACTCCCAATTTTATTTAAAA
CCATAACAATTAATTAGGAAGGGATTAAATAGCTGCCAATTTGATTTTGAGTCCACTACTTGTTCATGCCAGTCTTCTCCTTCACAAGATTAAC
AGCTCCTCCTGCGGTCTCCGATATCTTCTGTCCTGCGGTTTGAGCAGACGCTCCAGCAGCGGTAGCAGCATCCTTGACCTTGTCCACCAGCGCA
TTAGACTTCTCCTCAGCACGACCAGCGGCTTGACCGGCTTGGAAGCTTTGCTTGTTGTCTGCCATTTTTCTTAAGCAAATATTTTTGTTTATAT
GAATTTTGATGTGTTTCGTTTGATCC |
Primers for UN22220 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CAAACGTACAAACCTAAGGGGA |
60.262 |
22 |
CGCAGGAGGAGCTGTTAATC |
59.978 |
20 |
238 |
58 |
295 |
| 2 |
TCCAAACGTACAAACCTAAGGG |
60.262 |
22 |
CGCAGGAGGAGCTGTTAATC |
59.978 |
20 |
240 |
56 |
295 |
| 3 |
CAAACGTACAAACCTAAGGGGA |
60.262 |
22 |
AGAAGATATCGGAGACCGCA |
59.797 |
20 |
254 |
58 |
311 |
|
|
