 |
Simple sequence repeat (SSR)
>UN23721
GGCCTCACGTTAATAAAACTAAAATCAAACTCTGTAATCCAAAAGAGAATTTACACAATAGCATTAACAAAACAAAAAAAGGTCTCTCTAGTGG
CAAGTGAATTTTGAAATCTTGATTAAACAAAATACCAAAAGAATCTATACAGTGCTTTTCTTTCATCCAAAAGGTTCCATGAACAAAGATAATG
AACTTTCTTCTTGTTTGCAAGGTAACATCTTTGCTCTTGACTGAAGATATACACTTCTTTACTCAACCTTCACCTCTGTAAGATCATCTAAAAG
CTTCCTTGACGATGATGATGAACGTTTTTCTTCTTCTTCTTCTTCTTCTCGTTTCATGTCAACATCTTTGCCTGGCGCAGAGCACGAAGAGTCA
ACCCAAGGACCATCCATCGAGGACGAGCTTTTCTTTTCTCTGTCTCTCTCAAAGGTAGAAGCCTGTGACTCGCTGTCTTGTGACAAAGGCTCAA
AGGCCCTAAACCTCATTGCAGCTGCACCAGTTGGATCAGCCTCACGTTTCACCAAAGGTGGTTCCACTTTGCTTTTCCCTTCTAACATACTCAC
AACAGATGACATTGGTGGTCTTAACGTCGGAGATGGGTTTGTGCAGAGCAGAGCGATGTTCAACATCCTCTTTGCTTCTTTCTTGGAGAAGTTT
GTACCGAGATCCATGTCCACAAGCTCTAGTAGATTCCCTTGTTCTTGCAAGACATAGGCCCAATCAAGAAGGTAAACAAACTCTTCCTTTGGTC
TGTAGTTCGTGTTGCTCTTCCCGCTTACAATCTCTAAACAGACAACACCGAAGCTGTAGACGTCAGCTTTGTCTGTTAAGTAACCTCTCATTGC
GTACTCTGGAGCATGTACCTATTGTTCTGCATCTTGTGC |
Primers for UN23721 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
AAGCTTCCTTGACGATGATGA |
59.828 |
21 |
ACCTTTGGTGAAACGTGAGG |
60.005 |
20 |
250 |
280 |
529 |
| 2 |
AAGCTTCCTTGACGATGATGA |
59.828 |
21 |
GCGAGTCACAGGCTTCTACC |
60.02 |
20 |
170 |
280 |
449 |
| 3 |
AAGCTTCCTTGACGATGATGA |
59.828 |
21 |
CCTTTGTCACAAGACAGCGA |
60.025 |
20 |
186 |
280 |
465 |
|
|
