 |
Simple sequence repeat (SSR)
>UN25175
CGCCGGGGACGAACAAAAGCTCACAAAGAAGAGAAAACACAAAGAGAGAGAGAGAAGAAACAATCCAGCCGGAGAAGACGAAAAGAGAAGATGT
CGTGGCAATCATACGTCGATGACCATCTCCTGTGCGATGTGGAAGGCAGCCGCCTCACATCCGCCGCCATCCTCGGCCAAGACGGCAGTGTCTG
GGCCCAAAGCTCGAACTTCCCTCAGTTGAAGACTACTGAGATAGATGGAATCAGGAAAGACTTTGAGGAACCTGGGCATCTTGCCCCAACAGGG
CTCTTCCTTGGCGGAGAGAAGTACATGGTTATCCAAGGTGAAGCCGGAGCTGTCATCCGAGGCAAAAAGGGACCTGGTGGAGTCACTATCAAGA
AGACCAACCAAGCCTTTGTCTTTGGCATCTACGATGAACCAATGACTGGAGGTCAGTGCAACTTGGTGGTGGAGAGGCTCGGCGATTACCTTAT
CGAGTCTGATCTCTAAGTTTACACTCTTTCAAAAACGTGCTTCAAACCAAAATTTGAACTTTAAGGTTCTTCTTTTACTACATAAAACACTCTT
CTGCCGTGTATTATGATTTGATTTGTGGTCGTGCATCAAAAACAAAAACCTAAACTATACTCTTGAAAATGATCTAAGAAACAAATCACCCTTT
GTGTTTTTGTTTTGGAGTCTCTCTCTATTGTTCTTCTTGTGTTGTATTTCTTAATGTATCTCTCTTGTTTTCGATTTTAATAATAAAGTAACTC
CTGTTTGGTACAAAAA |
Primers for UN25175 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
GGACGAACAAAAGCTCACAA |
58.9 |
20 |
CGTATGATTGCCACGACATC |
59.955 |
20 |
103 |
7 |
109 |
| 2 |
GGACGAACAAAAGCTCACAA |
58.9 |
20 |
CATCGACGTATGATTGCCAC |
59.955 |
20 |
109 |
7 |
115 |
| 3 |
GGGACGAACAAAAGCTCACA |
61.216 |
20 |
CGTATGATTGCCACGACATC |
59.955 |
20 |
104 |
6 |
109 |
|
|
