 |
Simple sequence repeat (SSR)
>UN25625
GGAAGAACACTCCTCTTCTAATTGTGCTTATTCTCTCTCCTTCTCTCTCACTTTCTGTAGATTTATACACTGAAAATCAAGTTTCTGAGTTCAG
TTTCTGACAATGGAAGCAAACCTGTTATACGGTTTGTCTACACTTGTTGTTATTCTGTTATTGTCGGTGACTTCGACAGTGACATCAAAGGACG
ATGTTGTTTCCTGTACAATGTGTTCTTCCTGCGACAATCCTTGTAACCCCGTCCAGTCATATCCTCCCCCTCCGCCACCTTCTCGCCCACCACC
GTCTCCATCAACCACCACCGCATGTCCTCCACCTCCTTCTCCTCCTAGCTCCGGCGGTGGTGGTGGTGGTAGCTCTTACTATTACCCTCCTCCT
TCTCAGTCCGGTGGCGGAAAATACCCTCCTCCGTACGGTGACGGTGGTCAAGGTTATTACTATCCTCCTCCGTATTCAGGAAACTATCCTACTC
CCCCTCCGCCTAATCCGATCGTCCCTTACTTCCCGTTTTACTATCATACGCCACCACCAGGTTCTGGAGCAGATCGGTTTAAGGGTTCTAACTC
TATTCTGTTTGCTCTTTTCGCTGTCTTTCTCTGTTTAGTGTGACGGAAAATAGTCAGAGTTGGCCGGAAAATACCAAGTAATGTGGCAGATCGG
TAGATCTACAATAGTCTTTGAAGGTTTTAGATATCTGTGATGTTGGATCAAATGATTATCTCTTACTTTCTTTAACCTTTTCTTGTGTGGACAA
TTTGTATTTAATTTTTCTTGTTAATTAACATTTTGATTACTAGCTATTCAGAAATAAAGTATTGTTACTTTCTATGCATTATTTCTCTCTTTTT
AATGTATTGTATGGTTGATGTATTGCTCTTTAAGTGGTTTACACTCGAGTTATTTGTTCATCTCAGC |
Primers for UN25625 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CTCCACCTCCTTCTCCTCCT |
59.801 |
20 |
AACGGGAAGTAAGGGACGAT |
59.827 |
20 |
199 |
309 |
507 |
| 2 |
TTCTTCCTGCGACAATCCTT |
59.813 |
20 |
CCGTACGGAGGAGGGTATTT |
60.2 |
20 |
204 |
211 |
414 |
| 3 |
CTCCACCTCCTTCTCCTCCT |
59.801 |
20 |
CCGTACGGAGGAGGGTATTT |
60.2 |
20 |
106 |
309 |
414 |
|
|
