 |
Simple sequence repeat (SSR)
>UN25850
AAAACGAACTTTTTTTTATTATTACAATCTCAAATGAGTTCAAATCAGGCTTGGTTCCAATAATACTTACATTACATAAACGACTAACGAGGGG
AGCAATCAATCACCTGAGACCAACAAACTGCTAATTCTAAGACATGCCATACCCAACAGTGCCTATGCTCCTATGTGCTGCAACTGCAACCGCG
GCGGCTCCAGCAGGACCATACTGGGATTGTGCCTGCAACAGCTTAGCATCAATACCCACCTTGTGCTGTGATTGGACATAAGGGTTTGGGTTCA
GCTCAGCTGGCTTCGAGGAGTTGCATTGGGTTGGGACAACAAACTGCGGTTTTGGCTGGGAAGCAGCAGCTTCTGTACCACCACCACCACCACG
CTTCTCTTGGTTCATGGTGTTAGGATGGAAGAAACAGTTAGGAGATGCGGTCTGTTGAGAGGGTACCGTGCTTCTGTAATAAGAAGTCCTTGCA
TCATGAGACGACTCTTTGAGGTTCCTGGCATTCTCATAACTTACAGATGTTTCCACAGCACGGCCTGGTCTAGCTGATGGTACTCTTGGAGGAG
GTCCTAAAGGTTTAGTTGAAGCTGATGGAGTTACACCGTTTCTTGAAGGTTCGAAAGAAACACGTCTACTGTCTGGTTTGTTCAAGCCAGGTTG
AGCACTGGAGGCTACTACACTGGAGTGAACTGTAGACCGTGGAAGTGAAGTATGCTTTCTCTCTGGAGGTATGACTGGCCCAATTTTGCCGCTA
TTTTCTTCCAAGTAAGCAAACTGTTTCCTCAAAT |
Primers for UN25850 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CAGCAGGACCATACTGGGAT |
59.95 |
20 |
AGAAGCACGGTACCCTCTCA |
59.867 |
20 |
256 |
196 |
451 |
| 2 |
CCTGCAACAGCTTAGCATCA |
60.157 |
20 |
AGAAGCACGGTACCCTCTCA |
59.867 |
20 |
232 |
220 |
451 |
| 3 |
CAGCAGGACCATACTGGGAT |
59.95 |
20 |
GAAGCACGGTACCCTCTCAA |
60.255 |
20 |
255 |
196 |
450 |
|
|
