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Simple sequence repeat (SSR)
>UN25859
GGAAATCTCACTCCTCTTATCATTCTGTCCGTAGAGATTGATGTCCATGGAAGAGCTAAAACCTTCGAACAATAAGATTCTTGAGCAGCTGAGA
CATGGACTCGCTAGGTTCGAGCTTGTGTCCTCCCCTACTCTTTCAGTCTCCTCTGCTTCCTTCTTAGGCGCCACGCAGAGCAATAGCTATGCCT
TCTTCGCCAGAATCGGTTCCTCCTTAAATGGGTCTCCAGCAGCGAAGAAGGTTGAGCAGTACGCGGTGGATAGAGTTACTGGGGATGGTCGCTG
TCTCTTTAGAGCTTTGGTGAAAGGAATGGCCTTTAACAAAGGACTTACTATCAATCCCCGGAGAGAGAGAGATGATGCAGATGAACTACGAATG
GCTGTGAAAGAGGTTATATGCATTGACCCCAAGGAAAGGGAGAAGTACAAAGAAGCTTTAGTAGCTATCACTGTGGAAGAGTCTATTAAACGGT
ATTGTCAGCGGATTGGAAGACATGATTTCTGGGGAGGAGAGTCTGAGCTGCTTGTGCTTTCCAAGCTATGTAAACAGCCTATCATCGTCTACAT
ACCAGAGCATGAGCATAGGAGAGGAGGAGGGTATGGGGTCGGGGTTTATACCAATTGCAGAGTATGGAGCTGAGTTTAGAGGAGGCTGGGGAAA
AGGGAACAACAAGAAGAATGTTGTTAGGCTTCTTTACAGTGGTAGAAACCATTATGATCTGCTTCGATAGACCAAATAGATTGGAGTCTTGTAC
CTCTGTTTGAATCATCCATTATTCCAAATTTGTTTAAAACTTGGATTCGGTTTGGCTACTTTGGATCCAGCCAAACTTAATTACGTCTGGTTTG
GTTTCGTGTTTGGATTTAGTACGGTTTGGTTTGATTTGGTTACAAAGT |
Primers for UN25859 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
CCTTAAATGGGTCTCCAGCA |
60.066 |
20 |
CCTCTTTCACAGCCATTCGT |
60.255 |
20 |
180 |
210 |
389 |
| 2 |
CCTTAAATGGGTCTCCAGCA |
60.066 |
20 |
ACCTCTTTCACAGCCATTCG |
60.255 |
20 |
181 |
210 |
390 |
| 3 |
CGGTTCCTCCTTAAATGGGT |
60.18 |
20 |
CCTCTTTCACAGCCATTCGT |
60.255 |
20 |
188 |
202 |
389 |
|
|
