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Simple sequence repeat (SSR)
>UN25889
GGAGAAGGACTGATCCTTAGTACACGTGTCCTCTCGTTTTGTCCTCCGCGTCCTCTCTCTCAGATCACAAGATCCTGCATCCTCCGACACCATT
GTGTTTCGTTTCGTTCCTCGCCTTCTAGCCTTCGGCCTCATCACCGACTTCCTCTGGGCTATTGCTTTTGTCTTGTACCTCTTTAAAGAACCTC
ACTCACTCTCTCTTTCTCTCTGTTACCAAAAATCCCCAAATCTTGAAAAGTTTTAGCCTTTTAGGGTTTGAAGACTGAGAGAGAGAGATGAGTG
CTTCAAGGTTTATCAAGTGTGTAACAGTCGGCGACGGCGCTGTTGGAAAGACTTGTCTGCTCATCTCCTACACTAGCAACACTTTCCCCACGGC
CAGCTTCTTTAAAAAGGCTTCTCCTTTTCTCTGTTTTTCCTTCTTCTGGGTTTCTAAACACAAGAGAGGGATACGAAATGGTCCTAAGGCTCTT
AAGCTTGTTCCTGTCATCATCCGCTGCAGGAGTTGCGGGAGAGTTAAGCTGCCACATTTCTTCTGTTGCAGTGGTGAGCGGGGAAACCCTAGTG
AGCAAGGCAATTAGAATAAAGTCGAGTGATACTTTAGACATAGTTGACTCTGTTTTGTCAGATTATTTGTTTTTTTTTCCCTGCAAGATCACCA
CACTCATGGTTCTCTTGACAAGTTATATTATTGTCAATGCTTGTTGCAGAGTCTTGGCCAGCTCATTTATATATTGTGTGCCCTTAGACTCAAA
ACGAACAGCTACTTCTACAAAAGAGT |
Primers for UN25889 SSR
| Primer pair | Forward primer (5'-3') |
Reverse primer (5'-3') | Product |
|---|
| Sequence | Temperature | Size | Sequence | Temperature |
Size | Size | Start | End |
|---|
| 1 |
ACCGACTTCCTCTGGGCTAT |
60.096 |
20 |
TTTTAAAGAAGCTGGCCGTG |
60.368 |
20 |
254 |
137 |
390 |
| 2 |
CCTCTGGGCTATTGCTTTTG |
59.839 |
20 |
TTTTAAAGAAGCTGGCCGTG |
60.368 |
20 |
246 |
145 |
390 |
| 3 |
CGACTTCCTCTGGGCTATTG |
59.83 |
20 |
TTTTAAAGAAGCTGGCCGTG |
60.368 |
20 |
252 |
139 |
390 |
|
|
