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Simple sequence repeat (SSR)

>UN27144
ACAGTGACAGTTCCATCCCCTTCACTTTCCTGTCGGATCCAAACTTGGTGTCACGAGATTCAAGCAGCCATGGCAAGTCTTATGATGGCCACAC
CGTTTCCAGGATCTCTCACTCAATGCAAGAAAACGAATAACTTGTCTGTTCAGAGAGCCTTTAAGGTTACATGTATGCAGACACCTTTGGAGGA
GCTATACAACGTCAAAGTGGAAAGGAAAGTGTCAGAGAGACGGTTAGAGGAGCTAGGGGTTTCGAGATGGTCAGTTTGGAAGACTGGGAAATGC
AAACTGCCGTGGGACTGGCAGGTTGATCAGTTGGTTTACATTGAAGAAGGAGAGGTTCGTGTCGTCCCCGAAGGCAGCAAGAGGTTTATGCAGT
TTTTGGCTGGAGATCTTGTTAGGTACCCGAAATGGTTTGAGGCTGATCTTTTCTTCAACGCTCCTTACCGCTAACGGTATTGTTTCAAGGCCTA
TGGTGATGATTGATTCCAATGTAGAGTTTTTGAGTTTAGAGCCCCCATGAAAGTTTGCACTTGTGTTTGTGTTGTTGTTGTTGTTGTAGTCTTA
TGGATCTTTTTTGGGATGATAAAATAACAAAACAATTTGTCTTATTCTTCTTCAGAAATTTGTGTAACGCTTACATAAAGT

Primers for UN27144 SSR

Primer pairForward primer (5'-3') Reverse primer (5'-3')Product
SequenceTemperatureSizeSequenceTemperature SizeSizeStartEnd
1 CGAAATGGTTTGAGGCTGAT 60.074 20 CGTTACACAAATTTCTGAAGAAGAA 58.954 25 230 404 633
2 TTTTGAGTTTAGAGCCCCCA 59.679 20 CGTTACACAAATTTCTGAAGAAGAA 58.954 25 136 498 633
3 AGGCAGCAAGAGGTTTATGC 59.481 20 CGTTACACAAATTTCTGAAGAAGAA 58.954 25 280 354 633